Complete genomic sequence of the Lactobacillus temperate phage LF1

Bo Hyun Yoon, Hyo Ihl Chang

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Bacteriophage LF1, a newly isolated temperate phage from a mitomycin-C-induced lysate of wild type Lactobacillus fermentum, was found to contain a double-strand DNA of 42,606 base pairs (bp) with a G+C content of 45%. Bioinformatic analysis of the phage genome revealed 57 putative open reading frames (ORFs). The predicted protein products of ORFs were determined and described. According to morphological analysis by transmission electron microscopy (TEM), LF1 has an isometric head and a non-contractile tail, indicating that it belongs to the family Siphoviridae. The temperate phage LF1 has a good genetic mosaic relationship with ΦPYB5 in the packaging module. To our knowledge, this is first report of genomic sequencing and characterization of temperate phage LF1 from wild-type L. fermentum isolated from Kimchi in Korea.

    Original languageEnglish
    Pages (from-to)1909-1912
    Number of pages4
    JournalArchives of virology
    Volume156
    Issue number10
    DOIs
    Publication statusPublished - 2011 Oct

    ASJC Scopus subject areas

    • Virology

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