Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

Shelley F.J. Wickham; Alexander Auer; Jianghong Min; Nandhini Ponnuswamy; Johannes B. Woehrstein; Florian Schueder; Maximilian T. Strauss; Jörg Schnitzbauer; Bhavik Nathwani; Zhao Zhao; Steven D. Perrault; Jaeseung Hahn; Seungwoo Lee; Maartje M. Bastings; Sarah W. Helmig; Anne Louise Kodal; Peng Yin; Ralf Jungmann; William M. Shih

doi:10.1038/s41467-020-18910-x

Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

Shelley F.J. Wickham, Alexander Auer, Jianghong Min, Nandhini Ponnuswamy, Johannes B. Woehrstein, Florian Schueder, Maximilian T. Strauss, Jörg Schnitzbauer, Bhavik Nathwani, Zhao Zhao, Steven D. Perrault, Jaeseung Hahn, Seungwoo Lee, Maartje M. Bastings, Sarah W. Helmig, Anne Louise Kodal, Peng Yin, Ralf Jungmann, William M. Shih

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.

Original language	English
Article number	5768
Journal	Nature communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-18910-x
Publication status	Published - 2020 Dec
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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Wickham, S. F. J., Auer, A., Min, J., Ponnuswamy, N., Woehrstein, J. B., Schueder, F., Strauss, M. T., Schnitzbauer, J., Nathwani, B., Zhao, Z., Perrault, S. D., Hahn, J., Lee, S., Bastings, M. M., Helmig, S. W., Kodal, A. L., Yin, P., Jungmann, R., & Shih, W. M. (2020). Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard. Nature communications, 11(1), Article 5768. https://doi.org/10.1038/s41467-020-18910-x

Wickham, SFJ, Auer, A, Min, J, Ponnuswamy, N, Woehrstein, JB, Schueder, F, Strauss, MT, Schnitzbauer, J, Nathwani, B, Zhao, Z, Perrault, SD, Hahn, J, Lee, S, Bastings, MM, Helmig, SW, Kodal, AL, Yin, P, Jungmann, R & Shih, WM 2020, 'Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard', Nature communications, vol. 11, no. 1, 5768. https://doi.org/10.1038/s41467-020-18910-x

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abstract = "DNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.",

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AU - Woehrstein, Johannes B.

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AU - Nathwani, Bhavik

AU - Zhao, Zhao

AU - Perrault, Steven D.

AU - Hahn, Jaeseung

AU - Lee, Seungwoo

AU - Bastings, Maartje M.

AU - Helmig, Sarah W.

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AU - Jungmann, Ralf

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Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

Abstract

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ASJC Scopus subject areas

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