Composition Analysis of Airborne Microbiota in Outdoor and Indoor Based on Dust Separated by Micro-sized and Nano-sized

Jinho Yang, Ji Hoon Seo, Young Koo Jee, Yoon Keun Kim, Jong Ryeul Sohn

Research output: Contribution to journalArticlepeer-review

Abstract

Airborne microorganisms are associated with human health and awareness of the important influence of nano-sized extracellular vesicles (EVs) on health has risen. Thus, we analyzed the micro-sized microbes (m-MBs) and nano-sized microbial EVs (n-MEVs) of outdoor and indoor air through a field study in Seoul, Korea. We conducted 16S rDNA-based metagenomic analysis of m-MBs and n-MEVs in outdoor airborne dust (OAD), indoor airborne dust (IAD), and indoor dust from carpets (IDC). The dominant taxa in OAD were altered depending upon the outside environment, such as sunny, haze, and rainy. Also, dominant taxa in IAD and IDC were changed depending on the outside environment. In addition, there were differences of microbiome composition and diversity between the m-MB and n-MEV in OAD, IAD, and IDC. m-MB in OAD were more correlated with that of IDC, whereas n-MEVs in OAD were more related to those in IAD. Thus, indoor bioaerosols can be affected by different source according to bioaerosol size. Additionally, risk of bioaerosols can be different according to dominant taxa, and therefore we suggested that further study for risk of dominant taxa according to environments is necessary. We suggested that nano-sized microbial EVs should be included as parameters to manage air quality.

Original languageEnglish
Article number210231
JournalAerosol and Air Quality Research
Volume23
Issue number7
DOIs
Publication statusPublished - 2023 Jul

Bibliographical note

Funding Information:
This work was supported by MD Healthcare, Inc., Korea University, the BK21 Plus program, Semyung University, and the National Research Foundation of Korea (NRF) grant funded by the Korean Government Ministry of Science & ICT (NRF-2016M3A9B6901516). This research was also supported by the Ministry of Environment as "the Graduate school of Particulate matter specialization and Basic Science Research Program” through the NRF funded by the Ministry of Education (NRF-2021R1A6A3A01088686 and NRF-2021R1F1A1064097).

Publisher Copyright:
© The Author(s).

Keywords

  • Bioaerosol
  • Extracellular vesicles
  • Metagenomics
  • Microbiota
  • Nanoparticle

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

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