Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes

M. T. Le; H. Choi; M. K. Choi; D. T. Nguyen; J. H. Kim; H. G. Seo; S. Y. Cha; K. Seo; T. Chun; L. B. Schook; C. Park

doi:10.1111/tan.12017

Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes

M. T. Le, H. Choi, M. K. Choi, D. T. Nguyen, J. H. Kim, H. G. Seo, S. Y. Cha, K. Seo, T. Chun, L. B. Schook, C. Park

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.

Original language	English
Pages (from-to)	528-535
Number of pages	8
Journal	Tissue Antigens
Volume	80
Issue number	6
DOIs	https://doi.org/10.1111/tan.12017
Publication status	Published - 2012 Dec

Keywords

Direct sequencing
Haplotype
Major histocompatibility complex
Polymerase chain reaction
SLA-DQA
Swine
Swine leukocyte antigen

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Biochemistry
Genetics

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10.1111/tan.12017

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title = "Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes",

abstract = "We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.",

keywords = "Direct sequencing, Haplotype, Major histocompatibility complex, Polymerase chain reaction, SLA-DQA, Swine, Swine leukocyte antigen",

author = "Le, {M. T.} and H. Choi and Choi, {M. K.} and Nguyen, {D. T.} and Kim, {J. H.} and Seo, {H. G.} and Cha, {S. Y.} and K. Seo and T. Chun and Schook, {L. B.} and C. Park",

year = "2012",

month = dec,

doi = "10.1111/tan.12017",

language = "English",

volume = "80",

pages = "528--535",

journal = "Tissue Antigens",

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AU - Le, M. T.

AU - Choi, H.

AU - Choi, M. K.

AU - Nguyen, D. T.

AU - Kim, J. H.

AU - Seo, H. G.

AU - Cha, S. Y.

AU - Seo, K.

AU - Chun, T.

AU - Schook, L. B.

AU - Park, C.

PY - 2012/12

Y1 - 2012/12

N2 - We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.

AB - We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.

KW - Direct sequencing

KW - Haplotype

KW - Major histocompatibility complex

KW - Polymerase chain reaction

KW - SLA-DQA

KW - Swine

KW - Swine leukocyte antigen

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U2 - 10.1111/tan.12017

DO - 10.1111/tan.12017

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SN - 0001-2815

VL - 80

SP - 528

EP - 535

JO - Tissue Antigens

JF - Tissue Antigens

IS - 6

ER -

Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes

Abstract

Keywords

ASJC Scopus subject areas

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