Proteogenomics provide opportunities for proteomic validation of gene structures, genomic alterations and functional relevance of novel findings obtained from genomic data analysis. However, for effective proteogenomic data integration, an extensive proteome profiling, approaching the gene coverage of genomics data, is critical. Here we developed a multi-stage database search method for comprehensive proteomics data analysis to complement whole transcriptome sequencing data. The method utilizes two complementary database search engines, MS-GF+ and MODa/MODi, in tandem. The MS/MS data were first subjected to MS-GF+ database search (1st stage search) and the unidentified MS/MS data from the 1st stage search were subsequently analyzed with the combined use of MODa and MODi (2nd stage search), tools for blind and unrestrictive modification search, respectively. When combined with mPE-MMR, a tool for accurate and extensive precursor masses assignments to MS/MS data, the multi-stage method exhibited a significant increase in identified peptides, modified peptides, mutated peptides, identified proteins and coding genes, compared to a conventional single-stage method. With the increased coverage of proteome profile, the genomics and proteomics data obtained from the same gastric tumor tissue were effectively integrated as evidenced by proBAMsuite analysis results, which showed abundant examples of peptides uniquely mapped to genomic locations as well as increased coverages of exon-exon junctions and coding regions with the multi-stage search method.
Bibliographical noteFunding Information:
This work was supported by the Collaborative Genome Program for Fostering New Post-Genome Industry ( NRF-2017M3C9A5031597 ) and a project (NRF-2014R1A2A1A11054147) of National Research Foundation funded by the Korean Ministry of Science and ICT . X.W. and B.Z.’s work was supported by the National Cancer Institute (NCI) CPTAC award U24 CA210954 and the Cancer Prevention & Research Institutes of Texas (CPRIT) award RR160027 .
© 2017 Elsevier B.V.
Copyright 2018 Elsevier B.V., All rights reserved.
- Multi-stage database search
- Unidentified spectra
ASJC Scopus subject areas
- Condensed Matter Physics
- Physical and Theoretical Chemistry