Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea: Implications for freshwater ecosystem biomonitoring

Kyong In Suh, Jeong Mi Hwang, Yeon Jae Bae, Ji Hyoun Kang

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing baseline reference for phylogenetic analysis and aquatic ecosystem biomonitoring. We obtained 112 novel sequences of the barcode region of the mitochondrial DNA cytochrome c oxidase subunit I gene representing 11 families, 25 genera, and 43 species of mayfly (Insecta: Ephemeroptera) from South Korea. No species shared barcode sequences and all can be identified with barcodes with a possible exception of some species. Minimum levels of interspecific genetic distances ranged from 6.7 to 32.9% (mean: 23.7%), whereas average levels of intraspecific divergence was 3.7%. The latter value was inflated by the presence of very high divergences within some taxa. In fact, approximately 33.3% (15/45) of the species included two or more haplotype clusters showing greater than 5.0% sequence divergence and some values were as high as 32.9%. Many of the species with high intraspecific divergences are para- or polyphyletic and represent the possibility of species complexes. Our study suggests that type or topotype specimens should be sequenced to identify accurate barcoding clusters with morphological species concepts and also to determine the status of currently synonymized species.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalEntomological Research
Volume49
Issue number1
DOIs
Publication statusPublished - 2019 Jan

Bibliographical note

Funding Information:
This study was supported by the project on the Survey and discovery of Freshwater animal and plant of the Nakdonggang National Institute of Biological Resources (NNIBR 111-1301) to KIS and by a grant (NRF-2017R1C1B1010741) to JHK from the Basic Science Research Program, through the National Research Foundation of Korea (NRF), which is funded by the Ministry of Science, ICT & Future Planning.

Publisher Copyright:
© 2019 The Entomological Society of Korea and John Wiley & Sons Australia, Ltd

Keywords

  • DNA barcode
  • Ephemeroptera
  • Korea
  • larvae
  • mayfly

ASJC Scopus subject areas

  • Insect Science

Fingerprint

Dive into the research topics of 'Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea: Implications for freshwater ecosystem biomonitoring'. Together they form a unique fingerprint.

Cite this