Comprehensive mapping of Epithelial Na+ channel α expression in the mouse brain

Ha Kyeong Kim, Sang-Hyun Choi, Dong Hoon Kim, Yong Taek Jeong

Research output: Contribution to journalArticlepeer-review

Abstract

Epithelial sodium channel (ENaC) is responsible for regulating Na+ homeostasis. While its physiological functions have been investigated extensively in peripheral tissues, far fewer studies have explored its functions in the brain. Since our limited knowledge of ENaC’s distribution in the brain impedes our understanding of its functions there, we decided to explore the whole-brain expression pattern of the Scnn1a gene, which encodes the core ENaC complex component ENaCα. To visualize Scnn1a expression in the brain, we crossed Scnn1a-Cre mice with Rosa26-lsl-tdTomato mice. Brain sections were subjected to immunofluorescence staining using antibodies against NeuN or Myelin Binding Protein (MBP), followed by the acquisition of confocal images. We observed robust tdTomato fluorescence not only in the soma of cortical layer 4, the thalamus, and a subset of amygdalar nuclei, but also in axonal projections in the hippocampus and striatum. We also observed expression in specific hypothalamic nuclei. Contrary to previous reports, however, we did not detect significant expression in the circumventricular organs, which are known for their role in regulating Na+ balance. Finally, we detected fluorescence in cells lining the ventricles and in the perivascular cells of the median eminence. Our comprehensive mapping of Scnn1a-expressing cells in the brain will provide a solid foundation for further investigations of the physiological roles ENaC plays within the central nervous system.

Original languageEnglish
Pages (from-to)681-694
Number of pages14
JournalBrain Structure and Function
Volume229
Issue number3
DOIs
Publication statusPublished - 2024 Apr

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.

Keywords

  • Epithelial sodium channel
  • Immunohistochemistry
  • Neuroanatomy
  • Transgenic mouse

ASJC Scopus subject areas

  • Anatomy
  • General Neuroscience
  • Histology

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