Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin

Yongwoo Kwon; Jin Hee Hong; Sungsam Kang; Hojun Lee; Yonghyeon Jo; Ki Hean Kim; Seokchan Yoon; Wonshik Choi

doi:10.1038/s41467-022-35738-9

Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin

Yongwoo Kwon, Jin Hee Hong, Sungsam Kang, Hojun Lee, Yonghyeon Jo, Ki Hean Kim, Seokchan Yoon, Wonshik Choi

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required to secure clear optical access. Here we present a high-speed reflection matrix microscope using a light source with a wavelength of 1.3 μm to reduce tissue scattering and aberration. Furthermore, we develop a computational conjugate adaptive optics algorithm designed for the recorded reflection matrix to optimally compensate for the skull aberrations. These developments allow us to realize label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks to the depth of cortical layer 4 with a spatial resolution of 0.79 μm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.

Original language	English
Article number	105
Journal	Nature communications
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-35738-9
Publication status	Published - 2023 Dec

Bibliographical note

Funding Information:
This work is supported by the Institute for Basic Science (IBS-R023-D1) (Y.K., J.H.H., S.K., H.L., Y.J., S.Y., and W.C.) and the National Research Foundation (NRF) of Korea grant (NRF-2020R1A2C3009309) (K.H.K.).

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General
General Physics and Astronomy

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title = "Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin",

abstract = "Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required to secure clear optical access. Here we present a high-speed reflection matrix microscope using a light source with a wavelength of 1.3 μm to reduce tissue scattering and aberration. Furthermore, we develop a computational conjugate adaptive optics algorithm designed for the recorded reflection matrix to optimally compensate for the skull aberrations. These developments allow us to realize label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks to the depth of cortical layer 4 with a spatial resolution of 0.79 μm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.",

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AU - Jo, Yonghyeon

AU - Kim, Ki Hean

AU - Yoon, Seokchan

AU - Choi, Wonshik

N1 - Funding Information: This work is supported by the Institute for Basic Science (IBS-R023-D1) (Y.K., J.H.H., S.K., H.L., Y.J., S.Y., and W.C.) and the National Research Foundation (NRF) of Korea grant (NRF-2020R1A2C3009309) (K.H.K.). Publisher Copyright: © 2023, The Author(s).

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Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin

Abstract

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ASJC Scopus subject areas

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