Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin

Yongwoo Kwon, Jin Hee Hong, Sungsam Kang, Hojun Lee, Yonghyeon Jo, Ki Hean Kim, Seokchan Yoon, Wonshik Choi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required to secure clear optical access. Here we present a high-speed reflection matrix microscope using a light source with a wavelength of 1.3 μm to reduce tissue scattering and aberration. Furthermore, we develop a computational conjugate adaptive optics algorithm designed for the recorded reflection matrix to optimally compensate for the skull aberrations. These developments allow us to realize label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks to the depth of cortical layer 4 with a spatial resolution of 0.79 μm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.

Original languageEnglish
Article number105
JournalNature communications
Issue number1
Publication statusPublished - 2023 Dec

Bibliographical note

Funding Information:
This work is supported by the Institute for Basic Science (IBS-R023-D1) (Y.K., J.H.H., S.K., H.L., Y.J., S.Y., and W.C.) and the National Research Foundation (NRF) of Korea grant (NRF-2020R1A2C3009309) (K.H.K.).

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General
  • General Physics and Astronomy


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