Concurrent enhancement of CO2 fixation and productivities of omega-3 fatty acids and astaxanthin in Haematococcus pluvialis culture via calcium-mediated homeoviscous adaptation and biomineralization

Byung Sun Yu, Young Joon Sung, Hong Il Choi, Ranjna Sirohi, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Haematococcus pluvialis has attracted interest as a bio-platform for producing omega-3 fatty acids (ω-3 FA) and astaxanthin that have a great potential as anti-inflammatory drugs. This study aimed to concurrently enhance the CO2 fixation and the productivities of ω-3 FA and astaxanthin, which have been difficult to achieve because of the dissimilar culture methods for each goal, via calcium-mediated homeoviscous adaptation and biomineralization. As a result of 3 mM of Ca2+ addition, ω-3 FA content was improved by 31% due to Ca2+-induced homeoviscous adaptation. Biomineralization was promoted by the extracellular carbonic anhydrase, which resulted in 46.3% improvement in CO2 fixation. CaCO3 from the biomineralization was beneficially re-used in the H. pluvialis culture and triggered 178- and 522-fold increased biomass productivity and astaxanthin content, respectively, thanks to its anisotropic nature. The Ca2+-based productivity enhancement strategy was applied to large-scale culture which resulted improvement in overall bioprocess performance.

Original languageEnglish
Article number125720
JournalBioresource technology
Volume340
DOIs
Publication statusPublished - 2021 Nov

Bibliographical note

Funding Information:
This work was supported by “ Carbon to X Project ” ( #2020M3H7A1098295 ), the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea, a grant ( #NRF-2019R1A2C3009821/2020R1A5A1018052 ) from the National Research Foundation of Korea (NRF), and the Korea University Grant.

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • Astaxanthin
  • Biomineralization
  • Haematococcus pluvialis
  • Omega-3 fatty acids

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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