Conditioned media of ASC-17D Sertoli cells induce G1-growth arrest of DU145 human prostate cancer cells

Hyeog Kang, Sang Wook Kang, Sang Hyun Choi, Kwang Ho Lee, Boe Gwun Chun, Bon Hong Min

    Research output: Contribution to journalArticlepeer-review


    We studied the effects of ASC-17D rat Sertoli cell-conditioned media (rSCCM) on the proliferation of the DU145 prostate cancer cells. rSCCM was prepared from ASC-17D cells cultured in DMEM/F-12 serum-free media at a nonpermissive temperature of 40°C, which is the condition for the high expression of clusterin. We found that rSCCM could inhibit the proliferation of DU145 cells by arresting the cell cycle in the G1 phase in a dose-dependent manner. This growth arresting activity was abolished by boiling rSCCM for 5 min. The G1 growth-inhibiting activity of rSCCM was also detected in other prostate-originated cancer cells examined (i.e., LNCaP and PC-3) but not in other cells (ASC-17D, HepG2, SK-N-SH, and NIH3T3). Western blot analysis of partially purified growth inhibiting fractions with the clusterin antibody showed that the cytostatic factor in rSCCM was not clusterin. This cytostatic factor was semipurified by DEAE-Sepharose, ammonium sulfate precipitation, and Phenyl-Sepharose column chromatography, and was estimated to have a molecular weight of 88 kDa by Sephacryl S-300 gel filtration.

    Original languageEnglish
    Pages (from-to)508-514
    Number of pages7
    JournalJournal of Biochemistry and Molecular Biology
    Issue number5
    Publication statusPublished - 1998 Sept 30


    • Conditioned media
    • DU145 prostate cancer cell
    • Growth arrest
    • Sertoli cell

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology


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