Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells

Dahyun Hwang, Hyun A. Jo, Seonwook Hwang, Jeong Keun Kim, In Ho Kim, Young Hee Lim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background Strengthening of intestinal tight junctions provides an effective barrier from the external environment. Goblet cell-derived trefoil factor 3 (TFF3) increases transepithelial resistance by upregulating the expression of tight junction proteins. Oxyresveratrol (OXY) is a hydroxyl-substituted stilbene found in the roots, leaves, stems, and fruit of many plants and known to have various biological activities. In this study, we investigated the strengthening effect of OXY on intestinal tight junctions through stimulation of TFF production in goblet cells. Methods We prepared conditioned medium from LS 174T goblet cells treated with OXY (GCO-CM) and investigated the effect of GCO-CM on strengthening tight junctions of Caco-2 cells. The mRNA and protein expression levels of major tight junction components (claudin-1, occludin, and ZO-1) were measured by quantitative real-time PCR and western blotting, respectively. Transepithelial electric resistance (TEER) was measured using an ohm/V meter. Monolayer permeability was evaluated by paracellular transport of fluorescein isothiocyanate-dextran. Results OXY showed a strong antioxidant activity. It significantly increased the expression level of TFF3 in LS 174T goblet cells. GCO-CM prepared by treatment with 2.5, 5, and 10 μg/ml OXY did not show cytotoxicity in Caco-2 cells. GCO-CM increased the mRNA and protein expression levels of claudin-1, occludin, and ZO-1. It also significantly increased tight junction integrity and reduced permeability in a dose-dependent manner. Conclusion OXY stimulates the expression of TFF3 in goblet cells, which might increase the integrity of the intestinal tight junction barrier.

Original languageEnglish
Pages (from-to)280-286
Number of pages7
JournalBiomedicine and Pharmacotherapy
Volume85
DOIs
Publication statusPublished - 2017 Jan 1

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Masson SAS

Keywords

  • Goblet cell
  • Oxyresveratrol
  • TEER
  • TFF3
  • Tight junction

ASJC Scopus subject areas

  • Pharmacology

Fingerprint

Dive into the research topics of 'Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells'. Together they form a unique fingerprint.

Cite this