Confocal imaging of biological tissues using second harmonic generation

Beop Min Kim; Patrick Stoller; Karen Reiser; Jurgen Eichler; Ming Yan; Alexander Rubenchik; Luiz Da Silva

doi:10.1117/12.388066

Confocal imaging of biological tissues using second harmonic generation

Beop Min Kim, Patrick Stoller, Karen Reiser, Jurgen Eichler, Ming Yan, Alexander Rubenchik, Luiz Da Silva

Research output: Contribution to journal › Conference article › peer-review

15 Citations (Scopus)

Abstract

A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

Original language	English
Pages (from-to)	435-440
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3914
DOIs	https://doi.org/10.1117/12.388066
Publication status	Published - 2000
Externally published	Yes
Event	Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA Duration: 2000 Jan 22 → 2000 Jan 27

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.388066

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title = "Confocal imaging of biological tissues using second harmonic generation",

abstract = "A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.",

author = "Kim, {Beop Min} and Patrick Stoller and Karen Reiser and Jurgen Eichler and Ming Yan and Alexander Rubenchik and {Da Silva}, Luiz",

year = "2000",

doi = "10.1117/12.388066",

language = "English",

volume = "3914",

pages = "435--440",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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publisher = "SPIE",

note = "Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical ; Conference date: 22-01-2000 Through 27-01-2000",

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TY - JOUR

T1 - Confocal imaging of biological tissues using second harmonic generation

AU - Kim, Beop Min

AU - Stoller, Patrick

AU - Reiser, Karen

AU - Eichler, Jurgen

AU - Yan, Ming

AU - Rubenchik, Alexander

AU - Da Silva, Luiz

PY - 2000

Y1 - 2000

N2 - A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

AB - A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

UR - http://www.scopus.com/inward/record.url?scp=0033690259&partnerID=8YFLogxK

U2 - 10.1117/12.388066

DO - 10.1117/12.388066

M3 - Conference article

AN - SCOPUS:0033690259

SN - 0277-786X

VL - 3914

SP - 435

EP - 440

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical

Y2 - 22 January 2000 through 27 January 2000

ER -

Confocal imaging of biological tissues using second harmonic generation

Abstract

ASJC Scopus subject areas

UN SDGs

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