Abstract
The effect of water-soluble phospholipid polymer conjugated to a protein on conformational change during heat-quenched stress was investigated in this study. Well-defined pyridine disulfide end-functional poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was synthesized by well controlled atom transfer radical polymerization method. Synthesized PMPC was the site specifically conjugated to a protein, and the effect on conformational change during heat-quenched stress was estimated by circular dichroism and fluorescence study. As a result, a single phospholipid polymer chain conjugated to a protein give a great effect on conformational preservation in both secondary and tertiary structures even after the heat-quenced process. Moreover, even the conformational recovery which gave the completely reversible conformational preservation was observed in circular dichroism study. The resulting protein activity was also confirmed, and no significant decline was induced by heat-quenched stress.
| Original language | English |
|---|---|
| Pages (from-to) | 4859-4867 |
| Number of pages | 9 |
| Journal | Biomaterials |
| Volume | 30 |
| Issue number | 28 |
| DOIs | |
| Publication status | Published - 2009 Oct |
| Externally published | Yes |
Bibliographical note
Funding Information:This research was partially supported by the Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Agency.
Keywords
- Bioconjugation
- Conformation change
- Enzyme
- Phospholipid polymer
- Protein
ASJC Scopus subject areas
- Bioengineering
- Ceramics and Composites
- Biophysics
- Biomaterials
- Mechanics of Materials