Conformationally constrained analogues of diacylglycerol (DAG). 28. DAG-dioxolanones reveal a new additional interaction site in the C1b domain of PKCδ

Yongseok Choi, Yongmei Pu, Megan L. Peach, Ji Hye Kang, Nancy E. Lewin, Dina M. Sigano, Susan H. Garfield, Peter M. Blumberg, Victor E. Marquez

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Diacylglycerol (DAG) lactones have provided a powerful platform for structural exploration of the interactions between ligands and the C1 domains of protein kinase C (PKC). In this study, we report that DAG-dioxolanones, novel derivatives of DAG-lactones, exploit an additional point of contact (glutamine 27) in their binding with the C1b domain of PKCδ. Mutation of this point of contact to glutamate selectively impairs binding of the DAG-dioxolanones compared to that of the corresponding DAG-lactones (1200- to 3000-fold versus 35- to 55-fold, respectively). The differential response of this mutated C1b domain to the DAG-dioxolanones relative to the DAG-lactones provides a unique tool to probe the role of the C1b domain in PKCδ function, where the response to the DAG-lactones affords a positive control for retained function. Using this approach, we show that the C1b domain of PKCδ plays the predominant role in the translocation of PKCδ to the membrane in the presence of DAG.

    Original languageEnglish
    Pages (from-to)3465-3481
    Number of pages17
    JournalJournal of Medicinal Chemistry
    Volume50
    Issue number15
    DOIs
    Publication statusPublished - 2007 Jul 26

    ASJC Scopus subject areas

    • Molecular Medicine
    • Drug Discovery

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