Construction of a 3D mammary duct based on spatial localization of the extracellular matrix

Youngkyu Cho; Woo Kyung Moon; Hoe Suk Kim; Kyuhwan Na; Ji Hun Yang; Yang Hoon Huh; Jeong Ah Kim; Seok Chung; Su Hyun Lee

doi:10.1038/s41427-018-0089-3

Construction of a 3D mammary duct based on spatial localization of the extracellular matrix

Youngkyu Cho
, Woo Kyung Moon
, Hoe Suk Kim
, Kyuhwan Na
, Ji Hun Yang
, Yang Hoon Huh
, Jeong Ah Kim
, Seok Chung^*
, Su Hyun Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Extracellular matrix (ECM)-based hydrogels can serve as scaffolds in reconstruction of physiologically relevant three-dimensional (3D) in vitro models. Biocompatible or integrated hydrogels can be used to modulate ECM properties such as stiffness and composition for studies of cell−ECM interactions and morphogenesis. To this end, we developed a new type of spatially modified collagen type 1 hydrogel by convective addition of collagen type 1 solution. The matrix displayed properties that were distinct from those of a collagen type 1 hydrogel and recapitulated the morphology and function of mammary epithelium in a 3D microfluidic platform. In this ECM, mammary epithelial cells secreted laminin and exhibited self-assembly into a basement membrane. Thus, this spatially modified ECM offers biophysical features that can facilitate the construction of mammary epithelium and, by extension, that of various other epithelial types. Additionally, our reconstructed mammary duct can be used as an in vitro model for the study of early-stage breast cancer.

Original language	English
Pages (from-to)	970-981
Number of pages	12
Journal	NPG Asia Materials
Volume	10
Issue number	10
DOIs	https://doi.org/10.1038/s41427-018-0089-3
Publication status	Published - 2018 Oct 1

Bibliographical note

Funding Information:
The authors thank Nak Won Choi from the Korea Institute of Science and Technology for technical assistance in preparing the TRITC-conjugated COL1. This work was supported by National Research Foundation of Korea grants funded by the Korean government to S.H.L. (No. 2013R1A1A2058789) and S.C. (NRF-2017R1A2B3007701 and 2014M3A7B4052193).

Publisher Copyright:
© 2018, The Author(s).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Modelling and Simulation
General Materials Science
Condensed Matter Physics

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10.1038/s41427-018-0089-3

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title = "Construction of a 3D mammary duct based on spatial localization of the extracellular matrix",

abstract = "Extracellular matrix (ECM)-based hydrogels can serve as scaffolds in reconstruction of physiologically relevant three-dimensional (3D) in vitro models. Biocompatible or integrated hydrogels can be used to modulate ECM properties such as stiffness and composition for studies of cell−ECM interactions and morphogenesis. To this end, we developed a new type of spatially modified collagen type 1 hydrogel by convective addition of collagen type 1 solution. The matrix displayed properties that were distinct from those of a collagen type 1 hydrogel and recapitulated the morphology and function of mammary epithelium in a 3D microfluidic platform. In this ECM, mammary epithelial cells secreted laminin and exhibited self-assembly into a basement membrane. Thus, this spatially modified ECM offers biophysical features that can facilitate the construction of mammary epithelium and, by extension, that of various other epithelial types. Additionally, our reconstructed mammary duct can be used as an in vitro model for the study of early-stage breast cancer.",

author = "Youngkyu Cho and Moon, \{Woo Kyung\} and Kim, \{Hoe Suk\} and Kyuhwan Na and Yang, \{Ji Hun\} and Huh, \{Yang Hoon\} and Kim, \{Jeong Ah\} and Seok Chung and Lee, \{Su Hyun\}",

note = "Funding Information: The authors thank Nak Won Choi from the Korea Institute of Science and Technology for technical assistance in preparing the TRITC-conjugated COL1. This work was supported by National Research Foundation of Korea grants funded by the Korean government to S.H.L. (No. 2013R1A1A2058789) and S.C. (NRF-2017R1A2B3007701 and 2014M3A7B4052193). Publisher Copyright: {\textcopyright} 2018, The Author(s).",

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doi = "10.1038/s41427-018-0089-3",

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AU - Cho, Youngkyu

AU - Moon, Woo Kyung

AU - Kim, Hoe Suk

AU - Na, Kyuhwan

AU - Yang, Ji Hun

AU - Huh, Yang Hoon

AU - Kim, Jeong Ah

AU - Chung, Seok

AU - Lee, Su Hyun

N1 - Funding Information: The authors thank Nak Won Choi from the Korea Institute of Science and Technology for technical assistance in preparing the TRITC-conjugated COL1. This work was supported by National Research Foundation of Korea grants funded by the Korean government to S.H.L. (No. 2013R1A1A2058789) and S.C. (NRF-2017R1A2B3007701 and 2014M3A7B4052193). Publisher Copyright: © 2018, The Author(s).

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Extracellular matrix (ECM)-based hydrogels can serve as scaffolds in reconstruction of physiologically relevant three-dimensional (3D) in vitro models. Biocompatible or integrated hydrogels can be used to modulate ECM properties such as stiffness and composition for studies of cell−ECM interactions and morphogenesis. To this end, we developed a new type of spatially modified collagen type 1 hydrogel by convective addition of collagen type 1 solution. The matrix displayed properties that were distinct from those of a collagen type 1 hydrogel and recapitulated the morphology and function of mammary epithelium in a 3D microfluidic platform. In this ECM, mammary epithelial cells secreted laminin and exhibited self-assembly into a basement membrane. Thus, this spatially modified ECM offers biophysical features that can facilitate the construction of mammary epithelium and, by extension, that of various other epithelial types. Additionally, our reconstructed mammary duct can be used as an in vitro model for the study of early-stage breast cancer.

AB - Extracellular matrix (ECM)-based hydrogels can serve as scaffolds in reconstruction of physiologically relevant three-dimensional (3D) in vitro models. Biocompatible or integrated hydrogels can be used to modulate ECM properties such as stiffness and composition for studies of cell−ECM interactions and morphogenesis. To this end, we developed a new type of spatially modified collagen type 1 hydrogel by convective addition of collagen type 1 solution. The matrix displayed properties that were distinct from those of a collagen type 1 hydrogel and recapitulated the morphology and function of mammary epithelium in a 3D microfluidic platform. In this ECM, mammary epithelial cells secreted laminin and exhibited self-assembly into a basement membrane. Thus, this spatially modified ECM offers biophysical features that can facilitate the construction of mammary epithelium and, by extension, that of various other epithelial types. Additionally, our reconstructed mammary duct can be used as an in vitro model for the study of early-stage breast cancer.

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Construction of a 3D mammary duct based on spatial localization of the extracellular matrix

Abstract

Bibliographical note

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