Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Matthew A. Churgin; Liping He; John I. Murray; Christopher Fang-Yen

doi:10.1016/j.ymeth.2014.05.001

Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Matthew A. Churgin, Liping He, John I. Murray, Christopher Fang-Yen

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.

Original language	English
Pages (from-to)	431-436
Number of pages	6
Journal	Methods
Volume	68
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2014.05.001
Publication status	Published - 2014 Aug 1

Bibliographical note

Funding Information:
C.F.-Y. was supported by NIH, Ellison Medical Foundation , and the Alfred P. Sloan Research Foundation . J.I.M. was supported by the NIH ( GM083145 ), by the Penn Genome Frontiers Institute , and by a grant from the Pennsylvania Department of Health , which disclaims responsibility for any analyses, interpretations, or conclusions.

Funding Information:
Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs ( P40 OD010440 ).

Keywords

C. elegans
Heat shock
Lasers
Transgenes

ASJC Scopus subject areas

Molecular Biology
General Biochemistry,Genetics and Molecular Biology

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10.1016/j.ymeth.2014.05.001

Cite this

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abstract = "The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.",

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AU - Fang-Yen, Christopher

N1 - Funding Information: C.F.-Y. was supported by NIH, Ellison Medical Foundation , and the Alfred P. Sloan Research Foundation . J.I.M. was supported by the NIH ( GM083145 ), by the Penn Genome Frontiers Institute , and by a grant from the Pennsylvania Department of Health , which disclaims responsibility for any analyses, interpretations, or conclusions. Funding Information: Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs ( P40 OD010440 ).

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N2 - The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.

AB - The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.

KW - C. elegans

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KW - Lasers

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Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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