Abstract
Through the use of the recombinant bacterium TV 1061, containing a grpE-luxCDABE fusion, and a previously developed continuous toxicity monitoring system we were able to detect protein damaging toxicity in aqueous systems. This bacterial strain is responsive to the toxicity of protein-damaging agents and produces a bioluminescent response in their presence. The system uses the two-stage mini-bioreactors previously developed in our lab which allows stability even under the conditions that the toxicity greatly exceeded the sub-lethal level. The response of the strain under these conditions shows a unique signature when compared to previously tested strains.
Original language | English |
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Pages (from-to) | 185-196 |
Number of pages | 12 |
Journal | ACS Symposium Series |
Volume | 762 |
DOIs | |
Publication status | Published - 2000 |
Externally published | Yes |
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)