Continuous production of bioethanol using microalgal sugars extracted from Nannochloropsis gaditana

Ja Hyun Lee, Hee Uk Lee, Ju Hun Lee, Soo Kweon Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


We developed a continuous production process of bioethanol from sugars extracted from Nannochloropsis gaditana. To improve algal sugar production, the reaction conditions of acid-thermal hydrolysis were investigated based on five different types of acid and their concentrations (1-4%), and the loading ratio of solid/liquid (S/L). As a result, the maximum hydrolysis efficiency (92.82%) was achieved under 2% hydrochloric acid with 100 g/L biomass loading at 121 oC for 15 min. The hydrolysates obtained from N. gaditana were applied to the main medium of Bretthanomyces custersii H1-603 for bioethanol production. The maximum bioethanol production and yield by the microalgal hydrolysate were found to be 4.84 g/L and 0.37 g/g, respectively. In addition, the cell immobilization of B. custersii was carried out using sodium alginate, and the effect of the volume ratio of cell/sodium alginate on bioethanol productivity was investigated in a batch system. The optimal ratio was determined as 2 (v/v), and the immobilized cell beads were applied in the continuous stirred tank reactor (CSTR). Continuous ethanol production was performed using both free cells and immobilized cells at 1 L CSTR. In both groups, the maximum bioethanol production and yield were achieved at dilution rate of 0.04 h-1 (3.93 g/L and 0.3 g/g by free cell, and 3.68 g/L and 0.28 g/g by immobilized cell, respectively).

Original languageEnglish
JournalKorean Journal of Chemical Engineering
Publication statusAccepted/In press - 2018 Jan 1


  • Bioethanol
  • CSTR
  • Fermentation
  • Hydrolysis
  • Nannochloropsis gaditana

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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