Abstract
In this study, in order to develop a continuous production process of lactosucrose in a packed-bed reactor, Sterigmatomyces elviae ATCC 18894 was selected and mutated. The mutant strain of S. elviae showed 54.3% higher lactosucrose production than the wild type. Reaction conditions such as temperature, pH, substrate concentration and flow rate were also optimized. Under optimized reaction conditions (50°C, pH 6.0, 25% sucrose and 25% lactose as substrate, flow rate 1.2 ml/min), the maximum concentration of lactosucrose (192 g/l) was obtained. In a packed-bed reactor, continuous production of lactosucrose was performed using S. elviae mutant immobilized in calcium alginate, and about 180 g/l of lactosucrose production was achieved for 48 days.
Original language | English |
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Pages (from-to) | 1533-1537 |
Number of pages | 5 |
Journal | Journal of microbiology and biotechnology |
Volume | 17 |
Issue number | 9 |
Publication status | Published - 2007 Sept |
Keywords
- Continuous production
- Lactosucrose
- Packed-bed reactor
- Sterigmatomyces elviae mutant
- Whole-cell immobilization
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology