Contribution of quorum-sensing system to hexadecane degradation and biofilm formation in Acinetobacter sp. strain DR1

Y. S. Kang, W. Park

    Research output: Contribution to journalArticlepeer-review

    87 Citations (Scopus)

    Abstract

    Aims: To investigate roles of quorum-sensing (QS) system in Acinetobacter sp. strain DR1 and rifampicin-resistant variant (hereinafter DR1R).Methods and Results: The DR1 strain generated three putative acyl homoserine lactones (AHLs), while the DR1R produced only one signal and QS signal production was abrogated in the aqsI (LuxI homolog) mutant. The hexadecane-degradation and biofilm-formation capabilities of DR1, DR1R, and aqsI mutants were compared, along with their proteomic data. Proteomics analysis revealed that the AHL lactonase responsible for degrading QS signal was highly upregulated in both DR1R and aqsI mutant, also showed that several proteins, including ppGpp synthase, histidine kinase sensors, might be under the control of QS signalling. Interestingly, biofilm-formation and hexadecane-biodegradation abilities were reduced more profoundly in the aqsI mutant. These altered phenotypes of the aqsI mutant were restored via the addition of free wild-type cell supernatant and exogenous C12-AHL.Conclusions: The QS system in strain DR1 contributes to hexadecane degradation and biofilm formation.Significance and Impact of the Study: This is the first report to demonstrate that a specific QS signal appears to be a critical factor for hexadecane degradation and biofilm formation in Acinetobacter sp. strain DR1.

    Original languageEnglish
    Pages (from-to)1650-1659
    Number of pages10
    JournalJournal of Applied Microbiology
    Volume109
    Issue number5
    DOIs
    Publication statusPublished - 2010 Nov

    Keywords

    • Diesel
    • Lactonase
    • LuxI
    • LuxR
    • Proteomics
    • Quorum-sensing (QS)

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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