Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells

Sun Jong Kim; Seon Hye Cheon; Seung Jun Yoo; Jinie Kwon; Jong Hyuk Park; Chul Geun Kim; Kunsoo Rhee; Seungkwon You; Joo Yong Lee; Sung Il Roh; Hyun Soo Yoon

doi:10.1016/j.febslet.2004.12.024

Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells

Sun Jong Kim, Seon Hye Cheon, Seung Jun Yoo, Jinie Kwon, Jong Hyuk Park, Chul Geun Kim, Kunsoo Rhee, Seungkwon You, Joo Yong Lee, Sung Il Roh, Hyun Soo Yoon

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.

Original language	English
Pages (from-to)	534-540
Number of pages	7
Journal	FEBS Letters
Volume	579
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2004.12.024
Publication status	Published - 2005 Jan 17

Keywords

Differentiation and undifferentiation
Extracellular matrix molecule
Fibroblast growth factor signaling

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.12.024

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@article{d93b7e5a2d4346678fa58a6e29094385,

title = "Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells",

abstract = "Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.",

keywords = "Differentiation and undifferentiation, Extracellular matrix molecule, Fibroblast growth factor signaling",

author = "Kim, {Sun Jong} and Cheon, {Seon Hye} and Yoo, {Seung Jun} and Jinie Kwon and Park, {Jong Hyuk} and Kim, {Chul Geun} and Kunsoo Rhee and Seungkwon You and Lee, {Joo Yong} and Roh, {Sung Il} and Yoon, {Hyun Soo}",

note = "Funding Information: This study was supported by the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Korean Ministry of Science and Technology (code: M102KL010001-02K1201-00310, M102KL010001-03K1201-00610). ",

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doi = "10.1016/j.febslet.2004.12.024",

language = "English",

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AU - Kim, Sun Jong

AU - Cheon, Seon Hye

AU - Yoo, Seung Jun

AU - Kwon, Jinie

AU - Park, Jong Hyuk

AU - Kim, Chul Geun

AU - Rhee, Kunsoo

AU - You, Seungkwon

AU - Lee, Joo Yong

AU - Roh, Sung Il

AU - Yoon, Hyun Soo

N1 - Funding Information: This study was supported by the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Korean Ministry of Science and Technology (code: M102KL010001-02K1201-00310, M102KL010001-03K1201-00610).

PY - 2005/1/17

Y1 - 2005/1/17

N2 - Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.

AB - Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.

KW - Differentiation and undifferentiation

KW - Extracellular matrix molecule

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Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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