Controlled and targeted delivery of growth factors to biological environments is important for tissue regeneration. Polylactic acid (PLA) hydrogel microparticles are attractive carriers for the delivery of therapeutic cargoes based on their superior biocompatibility and biodegradability, uniform encapsulation of cargoes, and non-requirement of organic solvents during particle synthesis. In this study, we newly present controlled growth factor delivery utilizing PLA-based hydrogel microcarriers synthesized via degassed micromolding lithography (DML). Based on the direct gelation procedure from the single-phase aqueous precursor in DML, bovine serum albumin, a model protein of growth factor, and fibroblast growth factor were encapsulated into microparticles with uniform distribution. In addition, by tuning the monomer concentration and adding a hydrolytically stable crosslinker, the release of encapsulated cargoes was efficiently controlled and extended to 2 weeks. Finally, we demonstrated the biological activity of encapsulated FGF-2 in PLA-based microparticles using a fibroblast proliferation assay.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government Ministry of Science and ICT ( MSIT , Korea) ( NRF-2016R1A5A1010148) , and the Basic Science Research Program through the NRF funded by the Ministry of Education ( NRF-2019R1I1A1A01062350 ). This research was also supported by the Technology Innovation Program ( 20018111 , Development of super-fast multiplex technology for the examination of diagnosis of infectious disease and in-body response test) funded by the Ministry of Trade, Industry, & Energy ( MOTIE , Korea).
© 2022 Elsevier B.V.
- Controlled release
- Growth factor
ASJC Scopus subject areas
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry