Controlled growth factor delivery via a degradable poly(lactic acid) hydrogel microcarrier synthesized using degassed micromolding lithography

Wookyoung Jang; Seok Joon Mun; Soung Yon Kim; Ki Wan Bong

doi:10.1016/j.colsurfb.2022.113088

Controlled growth factor delivery via a degradable poly(lactic acid) hydrogel microcarrier synthesized using degassed micromolding lithography

Wookyoung Jang, Seok Joon Mun, Soung Yon Kim, Ki Wan Bong

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Controlled and targeted delivery of growth factors to biological environments is important for tissue regeneration. Polylactic acid (PLA) hydrogel microparticles are attractive carriers for the delivery of therapeutic cargoes based on their superior biocompatibility and biodegradability, uniform encapsulation of cargoes, and non-requirement of organic solvents during particle synthesis. In this study, we newly present controlled growth factor delivery utilizing PLA-based hydrogel microcarriers synthesized via degassed micromolding lithography (DML). Based on the direct gelation procedure from the single-phase aqueous precursor in DML, bovine serum albumin, a model protein of growth factor, and fibroblast growth factor were encapsulated into microparticles with uniform distribution. In addition, by tuning the monomer concentration and adding a hydrolytically stable crosslinker, the release of encapsulated cargoes was efficiently controlled and extended to 2 weeks. Finally, we demonstrated the biological activity of encapsulated FGF-2 in PLA-based microparticles using a fibroblast proliferation assay.

Original language	English
Article number	113088
Journal	Colloids and Surfaces B: Biointerfaces
Volume	222
DOIs	https://doi.org/10.1016/j.colsurfb.2022.113088
Publication status	Published - 2023 Feb

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government Ministry of Science and ICT ( MSIT , Korea) ( NRF-2016R1A5A1010148) , and the Basic Science Research Program through the NRF funded by the Ministry of Education ( NRF-2019R1I1A1A01062350 ). This research was also supported by the Technology Innovation Program ( 20018111 , Development of super-fast multiplex technology for the examination of diagnosis of infectious disease and in-body response test) funded by the Ministry of Trade, Industry, & Energy ( MOTIE , Korea).

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

Biodegradation
Controlled release
Growth factor
Hydrogel
Microparticle

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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Cite this

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title = "Controlled growth factor delivery via a degradable poly(lactic acid) hydrogel microcarrier synthesized using degassed micromolding lithography",

abstract = "Controlled and targeted delivery of growth factors to biological environments is important for tissue regeneration. Polylactic acid (PLA) hydrogel microparticles are attractive carriers for the delivery of therapeutic cargoes based on their superior biocompatibility and biodegradability, uniform encapsulation of cargoes, and non-requirement of organic solvents during particle synthesis. In this study, we newly present controlled growth factor delivery utilizing PLA-based hydrogel microcarriers synthesized via degassed micromolding lithography (DML). Based on the direct gelation procedure from the single-phase aqueous precursor in DML, bovine serum albumin, a model protein of growth factor, and fibroblast growth factor were encapsulated into microparticles with uniform distribution. In addition, by tuning the monomer concentration and adding a hydrolytically stable crosslinker, the release of encapsulated cargoes was efficiently controlled and extended to 2 weeks. Finally, we demonstrated the biological activity of encapsulated FGF-2 in PLA-based microparticles using a fibroblast proliferation assay.",

keywords = "Biodegradation, Controlled release, Growth factor, Hydrogel, Microparticle",

author = "Wookyoung Jang and Mun, {Seok Joon} and Kim, {Soung Yon} and Bong, {Ki Wan}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government Ministry of Science and ICT ( MSIT , Korea) ( NRF-2016R1A5A1010148) , and the Basic Science Research Program through the NRF funded by the Ministry of Education ( NRF-2019R1I1A1A01062350 ). This research was also supported by the Technology Innovation Program ( 20018111 , Development of super-fast multiplex technology for the examination of diagnosis of infectious disease and in-body response test) funded by the Ministry of Trade, Industry, & Energy ( MOTIE , Korea). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) funded by the Korean Government Ministry of Science and ICT ( MSIT , Korea) ( NRF-2016R1A5A1010148) , and the Basic Science Research Program through the NRF funded by the Ministry of Education ( NRF-2019R1I1A1A01062350 ). This research was also supported by the Technology Innovation Program ( 20018111 , Development of super-fast multiplex technology for the examination of diagnosis of infectious disease and in-body response test) funded by the Ministry of Trade, Industry, & Energy ( MOTIE , Korea). Publisher Copyright: © 2022 Elsevier B.V.

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N2 - Controlled and targeted delivery of growth factors to biological environments is important for tissue regeneration. Polylactic acid (PLA) hydrogel microparticles are attractive carriers for the delivery of therapeutic cargoes based on their superior biocompatibility and biodegradability, uniform encapsulation of cargoes, and non-requirement of organic solvents during particle synthesis. In this study, we newly present controlled growth factor delivery utilizing PLA-based hydrogel microcarriers synthesized via degassed micromolding lithography (DML). Based on the direct gelation procedure from the single-phase aqueous precursor in DML, bovine serum albumin, a model protein of growth factor, and fibroblast growth factor were encapsulated into microparticles with uniform distribution. In addition, by tuning the monomer concentration and adding a hydrolytically stable crosslinker, the release of encapsulated cargoes was efficiently controlled and extended to 2 weeks. Finally, we demonstrated the biological activity of encapsulated FGF-2 in PLA-based microparticles using a fibroblast proliferation assay.

AB - Controlled and targeted delivery of growth factors to biological environments is important for tissue regeneration. Polylactic acid (PLA) hydrogel microparticles are attractive carriers for the delivery of therapeutic cargoes based on their superior biocompatibility and biodegradability, uniform encapsulation of cargoes, and non-requirement of organic solvents during particle synthesis. In this study, we newly present controlled growth factor delivery utilizing PLA-based hydrogel microcarriers synthesized via degassed micromolding lithography (DML). Based on the direct gelation procedure from the single-phase aqueous precursor in DML, bovine serum albumin, a model protein of growth factor, and fibroblast growth factor were encapsulated into microparticles with uniform distribution. In addition, by tuning the monomer concentration and adding a hydrolytically stable crosslinker, the release of encapsulated cargoes was efficiently controlled and extended to 2 weeks. Finally, we demonstrated the biological activity of encapsulated FGF-2 in PLA-based microparticles using a fibroblast proliferation assay.

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Controlled growth factor delivery via a degradable poly(lactic acid) hydrogel microcarrier synthesized using degassed micromolding lithography

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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