Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis

Hyun Jeong Oh, Yoojin Shin, Seok Chung, Do Won Hwang, Dong Soo Lee

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The effective role of exosome delivering neurogenic microRNA (miRNA) enables to induce efficient differentiation process during neurogenesis. The microfludic system capable of visualizing the exosomal behavior such as secretion, migration, and uptake of individual exosomes can be used as a robust technique to understand the exosome-mediated change of cellular behavior. Here, we developed the exosome-tracing microfluidic system to visualize exosomal transport carrying the neurogenic miRNA from leading to neighboring cells, and found a new mode of exosome-mediated cell-non-autonomous neurogenesis. The miR-193a facilitated neurogenesis in F11 cells by blocking proliferation-related target genes. In addition to time-lapse live-cell imaging using microfluidics visualized the convective transport of exosomes from differentiated to undifferentiated cells. Individual exosomes containing miR-193a from differentiated donor cells were taken up by undifferentiated cells to lead them to neurogenesis. Induction of anti-miR-193a was sufficient to block neurogenesis in F11 cells. Inhibition of the exosomal production by manumycin-A and treatment of anti-miR-193a in the differentiated donor cells failed to induce neurogenesis in undifferentiated recipient cells. These findings indicate that exosomes of neural progenitors and neurogenic miRNA within these exosomes propagate cell-non-autonomous differentiation to neighboring progenitors, to delineate the roles of exosome mediating neurogenesis of population of homologous neural progenitor cells.

Original languageEnglish
Pages (from-to)82-94
Number of pages13
JournalBiomaterials
Volume112
DOIs
Publication statusPublished - 2017 Jan 1

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea ( HI14C0466 ), and funded by the Ministry of Health & Welfare, Republic of Korea ( HI14C3344 ), and funded by the Ministry of Health & Welfare, Republic of Korea ( HI14C1277 ), and National Research Foundation of Korea grant funded by the Korea government (MSIP) ( 2015M3C7A1028926 ), and supported by the Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (NRF-2015M3C1A3002152), and supported by BioNano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project (Grant Number H-GUARD_2014M3A6B2060524 ).

Publisher Copyright:
© 2016

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Cell-non-autonomous
  • Exosome imaging
  • MiR-193a
  • Microfluidic platform
  • Neurogenesis

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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