Abstract
Recent development of super-resolution fluorescence imaging technique such as stochastic optical reconstruction microscopy (STORM) and photoactived localization microscope (PALM) has brought us beyond the diffraction limits. It allows numerous opportunities in biology because vast amount of formerly obscured molecular structures, due to lack of spatial resolution, now can be directly observed. A drawback of fluorescence imaging, however, is that it lacks complete structural information. For this reason, we have developed a super-resolution multimodal imaging system based on STORM and full-field optical coherence microscopy (FF-OCM). FF-OCM is a type of interferometry systems based on a broadband light source and a bulk Michelson interferometer, which provides label-free and non-invasive visualization of biological samples. The integration between the two systems is simple because both systems use a wide-field illumination scheme and a conventional microscope. This combined imaging system gives us both functional information at a molecular level (∼20nm) and structural information at the sub-cellular level (∼1μm). For thick samples such as tissue slices, while FF-OCM is readily capable of imaging the 3D architecture, STORM suffer from aberrations and high background fluorescence that substantially degrade the resolution. In order to correct the aberrations in thick tissues, we employed an adaptive optics system in the detection path of the STORM microscope. We used our multimodal system to obtain images on brain tissue samples with structural and functional information.
Original language | English |
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Title of host publication | Multimodal Biomedical Imaging X |
Editors | Fred S. Azar, Xavier Intes |
Publisher | SPIE |
ISBN (Electronic) | 9781628414066 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Event | Multimodal Biomedical Imaging X - San Francisco, United States Duration: 2015 Feb 7 → … |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 9316 |
ISSN (Print) | 1605-7422 |
Conference
Conference | Multimodal Biomedical Imaging X |
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Country/Territory | United States |
City | San Francisco |
Period | 15/2/7 → … |
Bibliographical note
Publisher Copyright:© 2015 SPIE.
Keywords
- Correlative imaging
- FF-OCM
- Fullfield Optical Coherence Microscopy
- Multi-modal imaging
- STORM
- Stochastic optical reconstruction microscopy
- Super-resolution imaging
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging