Abstract
A novel coumarin-based fluorogenic probe bearing the 2-picoIyI unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu2+ over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu2+ ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu2+ in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu2+.
Original language | English |
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Pages (from-to) | 2008-2012 |
Number of pages | 5 |
Journal | Journal of the American Chemical Society |
Volume | 131 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2009 Feb 11 |
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
- Biochemistry
- Colloid and Surface Chemistry