Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo; Inseon Lee; Sung Gil Hong; Sunhyung An; Jinwoo Lee; Euichaul Oh; Jungbae Kim

doi:10.1016/j.cej.2015.04.057

Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Kie Moon Woo, Inseon Lee, Sung Gil Hong, Sunhyung An, Jinwoo Lee, Euichaul Oh, Jungbae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Carbonic anhydrase (CA), an enzyme converting CO₂ to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO₂ conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

Original language	English
Pages (from-to)	232-239
Number of pages	8
Journal	Chemical Engineering Journal
Volume	276
DOIs	https://doi.org/10.1016/j.cej.2015.04.057
Publication status	Published - 2015 Sept 5

Bibliographical note

Funding Information:
This work was supported by the ‘ International Collaborative R&D Program ’ (No. 20118510020020 ) and ‘ Energy Efficiency & Resources Core Technology Program ’ (No. 20142020200980 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grants, funded by the Korea government Ministry of Trade, Industry & Energy . This work was also supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ), and by the Nano·Material Technology Development Program ( 2014M3A7B4052193 ), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning .

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

Calcium carbonate
Carbon conversion and utilization
Carbonic anhydrase
Crosslinked chitosan coating
Enzyme immobilization and stabilization
Magnetic mesoporous silica

ASJC Scopus subject areas

Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Industrial and Manufacturing Engineering

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10.1016/j.cej.2015.04.057

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title = "Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion",

abstract = "Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.",

keywords = "Calcium carbonate, Carbon conversion and utilization, Carbonic anhydrase, Crosslinked chitosan coating, Enzyme immobilization and stabilization, Magnetic mesoporous silica",

author = "Woo, {Kie Moon} and Inseon Lee and Hong, {Sung Gil} and Sunhyung An and Jinwoo Lee and Euichaul Oh and Jungbae Kim",

note = "Funding Information: This work was supported by the {\textquoteleft} International Collaborative R&D Program {\textquoteright} (No. 20118510020020 ) and {\textquoteleft} Energy Efficiency & Resources Core Technology Program {\textquoteright} (No. 20142020200980 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grants, funded by the Korea government Ministry of Trade, Industry & Energy . This work was also supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ), and by the Nano·Material Technology Development Program ( 2014M3A7B4052193 ), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning . Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

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AU - An, Sunhyung

AU - Lee, Jinwoo

AU - Oh, Euichaul

AU - Kim, Jungbae

N1 - Funding Information: This work was supported by the ‘ International Collaborative R&D Program ’ (No. 20118510020020 ) and ‘ Energy Efficiency & Resources Core Technology Program ’ (No. 20142020200980 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grants, funded by the Korea government Ministry of Trade, Industry & Energy . This work was also supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ), and by the Nano·Material Technology Development Program ( 2014M3A7B4052193 ), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning . Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/9/5

Y1 - 2015/9/5

N2 - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

AB - Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.

KW - Calcium carbonate

KW - Carbon conversion and utilization

KW - Carbonic anhydrase

KW - Crosslinked chitosan coating

KW - Enzyme immobilization and stabilization

KW - Magnetic mesoporous silica

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Crosslinked chitosan coating on magnetic mesoporous silica with pre-adsorbed carbonic anhydrase for carbon dioxide conversion

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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