Carbonic anhydrase (CA), an enzyme converting CO2 to bicarbonate, was adsorbed into magnetically-separable spherical mesocellular siliceous foam (Mag-S-MCF). Then, chitosan was adsorbed onto the surface of Mag-S-MCF with pre-adsorbed enzymes, and further crosslinked via the glutaraldehyde treatment. The resulting composite materials, the crosslinked chitosan coating on Mag-S-MCF with pre-adsorbed CA (ADS-CA/CS-GA), could effectively prevent leaching of enzymes, showing no decrease of enzyme activity under shaking (200rpm) for 85days. ADS-CA/CS-GA also showed no activity decrease under recycled uses via facile magnetic separation, and could be successfully used for the biocatalytic CO2 conversion to bicarbonate, which was further utilized to generate calcium carbonate in the second batch reactor.
Bibliographical noteFunding Information:
This work was supported by the ‘ International Collaborative R&D Program ’ (No. 20118510020020 ) and ‘ Energy Efficiency & Resources Core Technology Program ’ (No. 20142020200980 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) Grants, funded by the Korea government Ministry of Trade, Industry & Energy . This work was also supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ), and by the Nano·Material Technology Development Program ( 2014M3A7B4052193 ), all of which are funded by the Korea government Ministry of Science, ICT & Future Planning .
© 2015 Elsevier B.V.
- Calcium carbonate
- Carbon conversion and utilization
- Carbonic anhydrase
- Crosslinked chitosan coating
- Enzyme immobilization and stabilization
- Magnetic mesoporous silica
ASJC Scopus subject areas
- Environmental Chemistry
- Chemical Engineering(all)
- Industrial and Manufacturing Engineering