Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine. In this article, Lee and his colleagues reported the successful production of HLA-homozygous SCNT-PSCs using MNCs from frozen/thawed donated cord blood in a public bank and frozen (vitrified) human oocytes donated after storage for 5 years. Homozygous SCNT-PSC-derived cells are highly immunocompatible with allogeneic HLA-matched immune cells. Therefore, we may suggest that this SCNT technology facilitates the establishment of the human HLA-matched SCNT-PSC bank.
Bibliographical noteFunding Information:
We thank Drs. Jung-Il Lee and Seung-Young Go for technical help for humanized mouse project. This research is supported by the Bio & Medical Technology Development Program ( 2015M3A9C6028961 , 2017M3A9F8072235 , and 2017M3A9C6061284 ) and Basic Science Research Program ( 2019R1A2C2005681 ) of NRF and MSICT of Republic of Korea.
© 2020 The Authors
- frozen cord blood cells
- frozen oocytes
- homozygous HLA
- pluripotent stem cells
- somatic cell nuclear transfer
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology