Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han, Kyung Chae Jeong, Jea Won Cho, Byung Cheon Jeong, Hyun Kyu Song, Jae Young Lee, Dong Hyuk Shin, Sangho Lee, Byung Il Lee

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

    Original languageEnglish
    Pages (from-to)1384-1388
    Number of pages5
    JournalFEBS Letters
    Volume586
    Issue number9
    DOIs
    Publication statusPublished - 2012 May 7

    Bibliographical note

    Funding Information:
    We thank the staff at the Photon Factory (Japan) beamlines 5A and NW12A for assistance with the collection of the synchrotron data. This work was supported by the Basic Science Research Program ( 2009-0064219 and 2009-0074586 ), the Mid-career Researcher Program ( 2011-0029294 ) and the Bio & Medical Technology Development Program ( 2011-0030032 ) through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Education, Science and Technology to S.L. and B.I.L.

    Keywords

    • DNA binding
    • DUF2666
    • PF2050
    • Pyrococcus furiosus

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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