Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

Ji Young Lee; Si Hoon Park; Byung Cheon Jeong; Hyun Kyu Song

doi:10.1107/S2053230X14016872

Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

Ji Young Lee, Si Hoon Park, Byung Cheon Jeong, Hyun Kyu Song

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0Å resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3121 or P3221, with unit-cell parameters a = b = 73.5, c = 83.6Å. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (V M) of 2.61Å³Da^-1 and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way.

Original language	English
Pages (from-to)	1252-1255
Number of pages	4
Journal	Acta Crystallographica Section:F Structural Biology Communications
Volume	70
DOIs	https://doi.org/10.1107/S2053230X14016872
Publication status	Published - 2014 Sept 1

Bibliographical note

Publisher Copyright:
© 2014 International Union of Crystallography.

Keywords

RNA degradation
Saccharomyces cerevisiae
Ski7
mRNA surveillance
nonstop decay

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S2053230X14016872

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title = "Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae",

abstract = "Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0{\AA} resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3121 or P3221, with unit-cell parameters a = b = 73.5, c = 83.6{\AA}. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (V M) of 2.61{\AA}3Da-1 and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way.",

keywords = "RNA degradation, Saccharomyces cerevisiae, Ski7, mRNA surveillance, nonstop decay",

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doi = "10.1107/S2053230X14016872",

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AU - Lee, Ji Young

AU - Park, Si Hoon

AU - Jeong, Byung Cheon

AU - Song, Hyun Kyu

PY - 2014/9/1

Y1 - 2014/9/1

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AB - Ski7 (superkiller protein 7) plays a critical role in the mRNA surveillance pathway. The C-terminal fragment of Ski7 (residues 520-747) from Saccharomyces cerevisiae was heterologously expressed in Escherichia coli and purified to homogeneity. It was successfully crystallized and preliminary X-ray data were collected to 2.0Å resolution using synchrotron radiation. The crystal belonged to a trigonal space group, either P3121 or P3221, with unit-cell parameters a = b = 73.5, c = 83.6Å. The asymmetric unit contains one molecule of the C-terminal fragment of Ski7 with a corresponding crystal volume per protein mass (V M) of 2.61Å3Da-1 and a solvent content of 52.8% by volume. The merging R factor is 6.6%. Structure determination by MAD phasing is under way.

KW - RNA degradation

KW - Saccharomyces cerevisiae

KW - Ski7

KW - mRNA surveillance

KW - nonstop decay

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JO - Acta Crystallographica Section:F Structural Biology Communications

JF - Acta Crystallographica Section:F Structural Biology Communications

ER -

Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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