Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens

Kyeong Kyu Kim; Kwang Yeon Hwang; Won Hee Jang; Hwanghee H. Lee; Ook Joon Yoo; Myung Un Choi; Se Won Suh

doi:10.1006/abbi.1993.1233

Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens

Kyeong Kyu Kim, Kwang Yeon Hwang, Won Hee Jang, Hwanghee H. Lee, Ook Joon Yoo, Myung Un Choi, Se Won Suh

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P4₁2₁2 (or P4₃2₁2), with unit cell dimensions of a = 82.01 (±0.06) Å and c = 145.44 (±0.08) Å. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (V_M) of 2.56 Å³/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 Å Bragg spacing when exposed to CuKα X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 Å Bragg spacing) have been collected from a native crystal.

Original language	English
Pages (from-to)	417-419
Number of pages	3
Journal	Archives of Biochemistry and Biophysics
Volume	302
Issue number	2
DOIs	https://doi.org/10.1006/abbi.1993.1233
Publication status	Published - 1993 May 1
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1006/abbi.1993.1233

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title = "Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens",

abstract = "Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P41212 (or P43212), with unit cell dimensions of a = 82.01 (±0.06) {\AA} and c = 145.44 (±0.08) {\AA}. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (VM) of 2.56 {\AA}3/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 {\AA} Bragg spacing when exposed to CuKα X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 {\AA} Bragg spacing) have been collected from a native crystal.",

author = "Kim, {Kyeong Kyu} and Hwang, {Kwang Yeon} and Jang, {Won Hee} and Lee, {Hwanghee H.} and Yoo, {Ook Joon} and Choi, {Myung Un} and Suh, {Se Won}",

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T1 - Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens

AU - Kim, Kyeong Kyu

AU - Hwang, Kwang Yeon

AU - Jang, Won Hee

AU - Lee, Hwanghee H.

AU - Yoo, Ook Joon

AU - Choi, Myung Un

AU - Suh, Se Won

PY - 1993/5/1

Y1 - 1993/5/1

N2 - Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P41212 (or P43212), with unit cell dimensions of a = 82.01 (±0.06) Å and c = 145.44 (±0.08) Å. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (VM) of 2.56 Å3/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 Å Bragg spacing when exposed to CuKα X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 Å Bragg spacing) have been collected from a native crystal.

AB - Large crystals of carboxylesterase from Pseudomonas fluorescens have been grown in the presence of dioxane using ammonium sulfate and lithium sulfate as precipitant. They belong to the tetragonal space group P41212 (or P43212), with unit cell dimensions of a = 82.01 (±0.06) Å and c = 145.44 (±0.08) Å. The presence of one carboxylesterase dimer in the asymmetric unit gives the crystal volume per protein mass (VM) of 2.56 Å3/Da and solvent fraction of 52.0% by volume. The crystals diffract to at least 2.3 Å Bragg spacing when exposed to CuKα X rays from a rotating anode generator. X-ray data (nearly complete to 2.6 Å Bragg spacing) have been collected from a native crystal.

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ER -

Crystallization and preliminary X-ray crystallographic analysis of carboxylesterase from pseudomonas fluorescens

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