Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

Kyeong Kyu Kim; Kwang Yeon Hwang; Hye Sung Jeon; Sangsoo Kim; Robert M. Sweet; Chul Hak Yang; Se Won Suh

doi:10.1016/0022-2836(92)90537-T

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

Kyeong Kyu Kim, Kwang Yeon Hwang, Hye Sung Jeon, Sangsoo Kim, Robert M. Sweet, Chul Hak Yang, Se Won Suh

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1·0 mm × 0·5 mm × 0·3 mm. The crystals belong to the monoclinic space group P2₁; with unit cell parameters a = 84·91 A ̊, b = 47·33 A ̊, c = 86·00 A ̊, and β = 116·09 °. And they diffract to about 1·6 Å upon exposure to synchroton X-rays. X-ray data have been collected to 2·2 Å Bragg spacing from a native crystal.

Original language	English
Pages (from-to)	1258-1262
Number of pages	5
Journal	Journal of Molecular Biology
Volume	227
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(92)90537-T
Publication status	Published - 1992 Oct 20
Externally published	Yes

Keywords

Lipase
Pseudomonas cepacia
X-ray crystallography
crystallization
preliminary X-ray analysis

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(92)90537-T

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@article{d7c16d101c274a5c86920b5b2b6d128d,

title = "Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia",

abstract = "Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1·0 mm × 0·5 mm × 0·3 mm. The crystals belong to the monoclinic space group P21; with unit cell parameters a = 84·91 A ̊, b = 47·33 A ̊, c = 86·00 A ̊, and β = 116·09 °. And they diffract to about 1·6 {\AA} upon exposure to synchroton X-rays. X-ray data have been collected to 2·2 {\AA} Bragg spacing from a native crystal.",

keywords = "Lipase, Pseudomonas cepacia, X-ray crystallography, crystallization, preliminary X-ray analysis",

author = "Kim, {Kyeong Kyu} and Hwang, {Kwang Yeon} and Jeon, {Hye Sung} and Sangsoo Kim and Sweet, {Robert M.} and Yang, {Chul Hak} and Suh, {Se Won}",

note = "Funding Information: This work was supported by a Korea Science and Engineering Foundation grant (890502). A travel fund from Pohang Light Source is also acknowledged. Beamline X12-C at Brookhaven National Laboratory{\textquoteright}s National Synchrotron Light Source is supported by the Office of Health and Environmental Research of the United States Department of Energy.",

year = "1992",

month = oct,

day = "20",

doi = "10.1016/0022-2836(92)90537-T",

language = "English",

volume = "227",

pages = "1258--1262",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "4",

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TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

AU - Kim, Kyeong Kyu

AU - Hwang, Kwang Yeon

AU - Jeon, Hye Sung

AU - Kim, Sangsoo

AU - Sweet, Robert M.

AU - Yang, Chul Hak

AU - Suh, Se Won

N1 - Funding Information: This work was supported by a Korea Science and Engineering Foundation grant (890502). A travel fund from Pohang Light Source is also acknowledged. Beamline X12-C at Brookhaven National Laboratory’s National Synchrotron Light Source is supported by the Office of Health and Environmental Research of the United States Department of Energy.

PY - 1992/10/20

Y1 - 1992/10/20

N2 - Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1·0 mm × 0·5 mm × 0·3 mm. The crystals belong to the monoclinic space group P21; with unit cell parameters a = 84·91 A ̊, b = 47·33 A ̊, c = 86·00 A ̊, and β = 116·09 °. And they diffract to about 1·6 Å upon exposure to synchroton X-rays. X-ray data have been collected to 2·2 Å Bragg spacing from a native crystal.

AB - Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1·0 mm × 0·5 mm × 0·3 mm. The crystals belong to the monoclinic space group P21; with unit cell parameters a = 84·91 A ̊, b = 47·33 A ̊, c = 86·00 A ̊, and β = 116·09 °. And they diffract to about 1·6 Å upon exposure to synchroton X-rays. X-ray data have been collected to 2·2 Å Bragg spacing from a native crystal.

KW - Lipase

KW - Pseudomonas cepacia

KW - X-ray crystallography

KW - crystallization

KW - preliminary X-ray analysis

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DO - 10.1016/0022-2836(92)90537-T

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AN - SCOPUS:0026462716

SN - 0022-2836

VL - 227

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EP - 1262

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

Abstract

Keywords

ASJC Scopus subject areas

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