Abstract
Large crystals of probable amylase/protease inhibitor-B have been grown at room temperature from ammonium sulfate solution. The crystals grow within five days to dimensions of 0.6 mm x 0.6 mm x 0.6 mm. They diffract to at least 1.7 AÅ upon exposure to synchrotron X-rays. The crystals belong to the space group P41212 (or P43212) with unit cell dimensions of a = 38.02 AÅ and c = 98.98 AÅ. The presence of one molecule per asymmetric unit gives the unit cell volume per protein mass (Vm) of 1.99 AÅ3/Da and the solvent fraction of 38.2% by volume. X-ray data have been collected to 2.0 AÅ Bragg spacing from native crystals.
Original language | English |
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Pages (from-to) | 255-257 |
Number of pages | 3 |
Journal | Journal of Molecular Biology |
Volume | 229 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1993 Jan 5 |
Externally published | Yes |
Keywords
- Crystallization
- Phospholipid transfer protein
- Possible amylase/protease inhibitor
- Preliminary X-ray analysis
- Rice seeds
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology