Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae

Ae Kyung Park, Seung Min Bong, Jin Ho Moon, Young Min Chi

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The response regulator DesR, which activates the transcription of the des gene by binding to a regulatory region, is essential for controlling the fluidity of membrane phospholipids. DesR from Streptococcus pneumoniae was overexpressed in Escherichia coli. The protein was purified and crystallized for structural analysis. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation and the crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 46.91, b = 71.38, c = 117.73 Å. Assuming the presence of a dimer in the asymmetric unit, this corresponds to a V M of 2.21 Å3 Da-1.

    Original languageEnglish
    Pages (from-to)727-729
    Number of pages3
    JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
    Volume65
    Issue number7
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Fatty-acid desaturation
    • Response regulators
    • Two-component systems

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Genetics
    • Condensed Matter Physics

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