Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

Kyeong Kyu Kim; Kwang Yeon Hwang; Kang Duk Choi; Joo Hyun Kang; Ook Joon Yoo; Se Won Suh

doi:10.1002/prot.340150212

Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

Kyeong Kyu Kim
, Kwang Yeon Hwang
, Kang Duk Choi
, Joo Hyun Kang
, Ook Joon Yoo
, Se Won Suh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 × 0.7 × 0.6 mm³ within a month. The crystals belong to the trigonal space group P3₁ (or P3₂), with unit cell dimensions of a= 147.12 Å and c= 131.08 Å. The asymmetric unit seems to contain six molecules of dimeric aryles‐terase, with corresponding crystal volume per protein mass (VM) of 2.53 Å³/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 Å Bragg spacing when exposed to X‐rays from a rotating‐anode source. X‐ray data have been collected to 2.9 Å Bragg spacing from native crystals. © 1993 Wiley‐Liss, Inc.

Original language	English
Pages (from-to)	213-215
Number of pages	3
Journal	Proteins: Structure, Function, and Bioinformatics
Volume	15
Issue number	2
DOIs	https://doi.org/10.1002/prot.340150212
Publication status	Published - 1993 Feb
Externally published	Yes

Keywords

X‐ray diffraction
bacterial esterase
crystals

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/prot.340150212

Cite this

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title = "Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens",

abstract = "Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 × 0.7 × 0.6 mm3 within a month. The crystals belong to the trigonal space group P31 (or P32), with unit cell dimensions of a= 147.12 {\AA} and c= 131.08 {\AA}. The asymmetric unit seems to contain six molecules of dimeric aryles‐terase, with corresponding crystal volume per protein mass (VM) of 2.53 {\AA}3/Da and solvent fraction of 51.5\% by volume. The crystals diffract to at least 2.2 {\AA} Bragg spacing when exposed to X‐rays from a rotating‐anode source. X‐ray data have been collected to 2.9 {\AA} Bragg spacing from native crystals. {\textcopyright} 1993 Wiley‐Liss, Inc.",

keywords = "X‐ray diffraction, bacterial esterase, crystals",

author = "Kim, \{Kyeong Kyu\} and Hwang, \{Kwang Yeon\} and Choi, \{Kang Duk\} and Kang, \{Joo Hyun\} and Yoo, \{Ook Joon\} and Suh, \{Se Won\}",

year = "1993",

month = feb,

doi = "10.1002/prot.340150212",

language = "English",

volume = "15",

pages = "213--215",

journal = "Proteins: Structure, Function, and Bioinformatics",

issn = "0887-3585",

publisher = "Wiley-Liss Inc.",

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T1 - Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

AU - Kim, Kyeong Kyu

AU - Hwang, Kwang Yeon

AU - Choi, Kang Duk

AU - Kang, Joo Hyun

AU - Yoo, Ook Joon

AU - Suh, Se Won

PY - 1993/2

Y1 - 1993/2

N2 - Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 × 0.7 × 0.6 mm3 within a month. The crystals belong to the trigonal space group P31 (or P32), with unit cell dimensions of a= 147.12 Å and c= 131.08 Å. The asymmetric unit seems to contain six molecules of dimeric aryles‐terase, with corresponding crystal volume per protein mass (VM) of 2.53 Å3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 Å Bragg spacing when exposed to X‐rays from a rotating‐anode source. X‐ray data have been collected to 2.9 Å Bragg spacing from native crystals. © 1993 Wiley‐Liss, Inc.

AB - Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 × 0.7 × 0.6 mm3 within a month. The crystals belong to the trigonal space group P31 (or P32), with unit cell dimensions of a= 147.12 Å and c= 131.08 Å. The asymmetric unit seems to contain six molecules of dimeric aryles‐terase, with corresponding crystal volume per protein mass (VM) of 2.53 Å3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 Å Bragg spacing when exposed to X‐rays from a rotating‐anode source. X‐ray data have been collected to 2.9 Å Bragg spacing from native crystals. © 1993 Wiley‐Liss, Inc.

KW - X‐ray diffraction

KW - bacterial esterase

KW - crystals

UR - https://www.scopus.com/pages/publications/0027522850

U2 - 10.1002/prot.340150212

DO - 10.1002/prot.340150212

M3 - Article

C2 - 8441756

AN - SCOPUS:0027522850

SN - 0887-3585

VL - 15

SP - 213

EP - 215

JO - Proteins: Structure, Function, and Bioinformatics

JF - Proteins: Structure, Function, and Bioinformatics

IS - 2

ER -

Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

Abstract

Keywords

ASJC Scopus subject areas

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