Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Dongmin Lee; Hyung Bae Kwon

doi:10.1016/j.jneumeth.2021.109407

Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Dongmin Lee, Hyung Bae Kwon

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Oxytocin is a neuropituitary hormone that is involved in a wide range of psychosocial behaviors. Despite its psychophysiological importance as a neuromodulator in the CNS, effective techniques capable of monitoring oxytocin dynamics or testing related behavioral consequences are limited. Along with an explosive advancement in synthetic biology, high-performance genetically-encoded neuromodulator sensors are being developed. Here we comprehensively review the current methodologies available for detecting oxytocin in neuroscience. Their strengths and weaknesses are discussed, and a graphical summary is plotted for better comparison of techniques. We also suggest future directions for next generation oxytocin sensor development and their working principles.

Original language	English
Article number	109407
Journal	Journal of Neuroscience Methods
Volume	366
DOIs	https://doi.org/10.1016/j.jneumeth.2021.109407
Publication status	Published - 2022 Jan 15

Keywords

Arrestin
Circularly permuted fluorescent protein
Enzyme complementation
Fast-scan cyclic voltammetry
G protein-coupled receptor
Gene expression
Genetically encoded sensor
Microdialysis
Neuromodulator
Neuropeptide
Oxytocin
Split luciferase
TEV protease

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.jneumeth.2021.109407

Cite this

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title = "Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors",

abstract = "Oxytocin is a neuropituitary hormone that is involved in a wide range of psychosocial behaviors. Despite its psychophysiological importance as a neuromodulator in the CNS, effective techniques capable of monitoring oxytocin dynamics or testing related behavioral consequences are limited. Along with an explosive advancement in synthetic biology, high-performance genetically-encoded neuromodulator sensors are being developed. Here we comprehensively review the current methodologies available for detecting oxytocin in neuroscience. Their strengths and weaknesses are discussed, and a graphical summary is plotted for better comparison of techniques. We also suggest future directions for next generation oxytocin sensor development and their working principles.",

keywords = "Arrestin, Circularly permuted fluorescent protein, Enzyme complementation, Fast-scan cyclic voltammetry, G protein-coupled receptor, Gene expression, Genetically encoded sensor, Microdialysis, Neuromodulator, Neuropeptide, Oxytocin, Split luciferase, TEV protease",

author = "Dongmin Lee and Kwon, {Hyung Bae}",

note = "Funding Information: This work was supported by National Research Foundation of Korea (NRF) grants; the Korea Government Ministry of Science and Information and Communications Technology (ICT) ( NRF‐2018M3C7A1024597 and NRF-2020R1C1C1014793 to D.L.), and by National Institute of Mental Health DP1MH119428 (to H-B.K). Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2022",

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doi = "10.1016/j.jneumeth.2021.109407",

language = "English",

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T2 - Focusing on genetically-encoded GPCR sensors

AU - Lee, Dongmin

AU - Kwon, Hyung Bae

N1 - Funding Information: This work was supported by National Research Foundation of Korea (NRF) grants; the Korea Government Ministry of Science and Information and Communications Technology (ICT) ( NRF‐2018M3C7A1024597 and NRF-2020R1C1C1014793 to D.L.), and by National Institute of Mental Health DP1MH119428 (to H-B.K). Publisher Copyright: © 2021 Elsevier B.V.

PY - 2022/1/15

Y1 - 2022/1/15

N2 - Oxytocin is a neuropituitary hormone that is involved in a wide range of psychosocial behaviors. Despite its psychophysiological importance as a neuromodulator in the CNS, effective techniques capable of monitoring oxytocin dynamics or testing related behavioral consequences are limited. Along with an explosive advancement in synthetic biology, high-performance genetically-encoded neuromodulator sensors are being developed. Here we comprehensively review the current methodologies available for detecting oxytocin in neuroscience. Their strengths and weaknesses are discussed, and a graphical summary is plotted for better comparison of techniques. We also suggest future directions for next generation oxytocin sensor development and their working principles.

AB - Oxytocin is a neuropituitary hormone that is involved in a wide range of psychosocial behaviors. Despite its psychophysiological importance as a neuromodulator in the CNS, effective techniques capable of monitoring oxytocin dynamics or testing related behavioral consequences are limited. Along with an explosive advancement in synthetic biology, high-performance genetically-encoded neuromodulator sensors are being developed. Here we comprehensively review the current methodologies available for detecting oxytocin in neuroscience. Their strengths and weaknesses are discussed, and a graphical summary is plotted for better comparison of techniques. We also suggest future directions for next generation oxytocin sensor development and their working principles.

KW - Arrestin

KW - Circularly permuted fluorescent protein

KW - Enzyme complementation

KW - Fast-scan cyclic voltammetry

KW - G protein-coupled receptor

KW - Gene expression

KW - Genetically encoded sensor

KW - Microdialysis

KW - Neuromodulator

KW - Neuropeptide

KW - Oxytocin

KW - Split luciferase

KW - TEV protease

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DO - 10.1016/j.jneumeth.2021.109407

M3 - Article

C2 - 34763021

AN - SCOPUS:85118836238

SN - 0165-0270

VL - 366

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

M1 - 109407

ER -

Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Abstract

Keywords

ASJC Scopus subject areas

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