De novo biosynthesis of 2ʹ-fucosyllactose by bioengineered Corynebacterium glutamicum

Ye Gi Lee, Hae Yong Jo, Do Haeng Lee, Jong Won Yoon, Young Ha Song, Dae Hyuk Kweon, Kyoung Heon Kim, Yong Cheol Park, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


2ʹ-Fucosyllactose (2ʹ-FL) which is well-known human milk oligosaccharide was biotechnologically synthesized using engineered Corynebacterium glutamicum, a GRAS microbial workhorse. By construction of the complete de novo pathway for GDP-L-fucose supply and heterologous expression of Escherichia coli lactose permease and Helicobacter pylori α-1,2-fucosyltransferase, bioengineered C. glutamicum BCGW_TL successfully biosynthesized 0.25 g L−1 2ʹ-FL from glucose. The additional genetic perturbations including the expression of a putative 2ʹ-FL exporter and disruption of the chromosomal pfkA gene allowed C. glutamicum BCGW_cTTLEΔP to produce 2.5 g L−1 2ʹ-FL batchwise. Finally, optimized fed-batch cultivation of the BCGW_cTTLEΔP using glucose, fructose, and lactose resulted in 21.5 g L−1 2ʹ-FL production with a productivity of 0.12 g L−1•h, which were more than 3.3 times higher value relative to the batch culture of the BCGW_TL. Conclusively, it would be a groundwork to adopt C. glutamicum for biotechnological production of other food additives including human milk oligosaccharides.

Original languageEnglish
Article number2300461
JournalBiotechnology Journal
Issue number1
Publication statusPublished - 2024 Jan

Bibliographical note

Publisher Copyright:
© 2023 Wiley-VCH GmbH.


  • 2ʹ-FL exporter
  • 2ʹ-fucosyllactose
  • Corynebacterium glutamicum
  • fed-batch cultivation
  • α−1,2-fucosyltransferase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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