Abstract
Remazol brilliant blue R (RBBR) is an anthraquinone dye derived from anthracene that is decolorized by a white rot fungus, Phlebia brevispora. Interestingly, P. brevispora produces two phenomena of yellowish and pinkish colors during the degradation of RBBR. Here, we characterized the decolorization of RBBR by P. brevispora. The fungus was significantly different between the two colors via UV spectrophotometry, and the morphology of the hyphae observed in the respective color culture was also entirely different. Moreover, both of the two ligninolytic enzymes, laccase and manganese-dependent peroxidase (MnP), were remarkably stimulated in the yellowish culture at the beginning of the decolorization. It is possible that the RBBR decolorizing mechanism might be primarily related to the amount of laccase and MnP produced in the yellowish culture. Thus, the decolorized color may be rapidly estimated at initial period of incubation. In addition, GeneFishing technology revealed that two genes were differentially expressed in yellowish culture.
| Original language | English |
|---|---|
| Pages (from-to) | 125-131 |
| Number of pages | 7 |
| Journal | Engineering in Life Sciences |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2017 Feb 1 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP) (NRF-2013R1A2A2A01068649); the “Oil Spill Environmental Impact Assessment and Environmental Restoration” (PM57431) funded by the Ministry of Oceans and Fisheries of Korea; and a Korea University Grant.
Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords
- Differentially expressed gene
- Ligninolytic enzyme
- Phlebia brevispora KUC9045
- Remazol brilliant blue R
- White rot fungi
ASJC Scopus subject areas
- Biotechnology
- Environmental Engineering
- Bioengineering