Deep optical imaging within complex scattering media

Seokchan Yoon, Moonseok Kim, Mooseok Jang, Youngwoon Choi, Wonjun Choi, Sungsam Kang, Wonshik Choi

Research output: Contribution to journalReview articlepeer-review

145 Citations (Scopus)


Optical imaging has had a central role in elucidating the underlying biological and physiological mechanisms in living specimens owing to its high spatial resolution, molecular specificity and minimal invasiveness. However, its working depth for in vivo imaging is extremely shallow, and thus reactions occurring deep inside living specimens remain out of reach. This problem originates primarily from multiple light scattering caused by the inhomogeneity of tissue obscuring the desired image information. Adaptive optical microscopy, which minimizes the effect of sample-induced aberrations, has to date been the most effective approach to addressing this problem, but its performance has plateaued because it can suppress only lower-order perturbations. To achieve an imaging depth beyond this conventional limit, there is increasing interest in exploiting the physics governing multiple light scattering. New approaches have emerged based on the deterministic measurement and/or control of multiple-scattered waves, rather than their stochastic and statistical treatment. In this Review, we provide an overview of recent developments in this area, with a focus on approaches that achieve a microscopic spatial resolution while remaining useful for in vivo imaging, and discuss their present limitations and future prospects.

Original languageEnglish
Pages (from-to)141-158
Number of pages18
JournalNature Reviews Physics
Issue number3
Publication statusPublished - 2020 Mar 1

Bibliographical note

Funding Information:
This research was supported by the Institute for Basic Science (grant no. IBS-R023-D1), the National Research Foundation of Korea (grant nos. NRF-2019R1C1C1008175 and NRF-2016R1A6A3A11936389), and the Catholic Medical Center Research Foundation in the programme year of 2018.

Publisher Copyright:
© 2020, Springer Nature Limited.

ASJC Scopus subject areas

  • Physics and Astronomy(all)


Dive into the research topics of 'Deep optical imaging within complex scattering media'. Together they form a unique fingerprint.

Cite this