Degradation of 2,4,6-trinitrotoluene by immobilized horseradish peroxidase and electrogenerated peroxide

Ki Beom Lee, Man Bock Gu, Seung Hyeon Moon

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


This paper presents horseradish peroxidase (HRP)-catalyzed removal of 2,4,6-trinitrotoluene (TNT) by an electrochemical packed-bed flow reactor operated in a circulating batch mode with the help of in situ generated hydrogen peroxide. HRP immobilized on the reticulated vitreous carbon electrode was prepared for the cyclic voltammetry of 2,4,6-TNT. Effects of pH and temperature on the TNT electroreduction in 0.2 M phosphate buffer saturated with oxygen were examined. HRP immobilized carbon electrode was capable of catalyzing the oxidation and detoxification of 44 μM TNT in aqueous solution under optimized conditions. The removal rate of TNT for the electroenzymatic method was much greater than for electrochemical and biochemical methods. Stoichiometric and kinetic studies indicated that the hydrogen peroxide was utilized more effectively in the electroenzymatic method. Denitrification as intermediate reaction was also investigated.

Original languageEnglish
Pages (from-to)983-992
Number of pages10
JournalWater Research
Issue number5
Publication statusPublished - 2003 Mar
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by Brain Korea 21 from the Ministry of Education through the Graduate Program for Chemical & Environmental Engineering at Kwangju Institute of Science & Technology.


  • 2,4,6-Trinitrotoluene
  • Cyclic voltammetry
  • Electroenzymatic method
  • Horseradish peroxidase

ASJC Scopus subject areas

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution


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