Degradation of Pyrene Contaminated Soil with Spiked 14C Pyrene by Hemoglobin Catalysis

Guyoung Kang, Sungjong Lee, Haein Keum, Namhyun Chung

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Hemoglobin (Hb) is a member of the hemeprotein family that undergoes non-specific catalytic chain reactions in the presence of hydrogen peroxide (H2O2). The catalytic ability of Hb to degrade the carcinogenic polycyclic aromatic hydrocarbon pyrene was demonstrated using soil contaminated with 14C pyrene. Three bench-scale laboratory tests were performed using 14C pyrene in the presence of a buffer, H2O2, and a combination of Hb and H2O2. The initial pyrene concentration of the contaminated soil was set to 11 mg/kg, with 5,500,000 dpm of 14C pyrene. After a catalytic reaction for 24 h, the results showed that 17% of pyrene was degraded by H2O2, 38% of pyrene was degraded by Hb in combination with H2O2, and 0.13 and 1.2% of 14C pyrene were mineralized by H2O2 and Hb in combination with H2O2, respectively. An analysis of the products from the reaction involving Hb in combination with H2O2 revealed that 15.9% of the 14C intermediates in the acetonitrile fraction were polar products. After the catalytic reaction, 21 intermediate compounds were found via fraction analysis. The results suggested that Hb catalysis could be used to treat pyrene-contaminated soil as a novel catalytic technology for the remediation of hazardous materials in soil.

Original languageEnglish
Pages (from-to)1665-1671
Number of pages7
JournalTopics in Catalysis
Issue number15-17
Publication statusPublished - 2018 Oct 1

Bibliographical note

Funding Information:
Acknowledgements This study was supported by the Hankuk University of Foreign Studies (2017).

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.


  • C pyrene
  • Hemoglobin
  • Hydrogen peroxide
  • Mass balance
  • Remediation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)


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