Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose

Chan Young Shin, Ji Woong Choi, Eun Sook Jang, Chung Ju, Won Ki Kim, Hyoung Chun Kim, Chang Rak Choi, Kwang Ho Ko

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Pretreatment of interferon-γ and lipopolysaccharides made C6 glioma cells highly vulnerable to glucose deprivation. Neither 12 h of glucose deprivation nor 2-day treatment with interferon-γ (100 U/ml) and lipopolysaccharides (1 μg/ml) altered the viability of C6 glioma cells. However, significant death of immunostimulated C6 glioma cells was observed after 5 h of glucose deprivation. The augmented death was prevented by dehydroepiandrosterone (DHEA) treatment during immunostimulation, but not by DHEA treatment during glucose deprivation. DHEA reduced the rise in nitrotyrosine immunoreactivity, a marker of peroxynitrite, and superoxide production in glucose-deprived immunostimulated C6 glioma cells. DHEA, however, did not protect glucose-deprived C6 glioma cells from the exogenously produced peroxynitrite by 3-morpholinosydnonimine. Further, DHEA did not alter the production of total reactive oxygen species and nitric oxide in immunostimulated C6 glioma cells. Superoxide dismutase (SOD) and the synthetic SOD mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin inhibited the death of glucose-deprived immunostimulated C6 glioma cells. In addition, a superoxide anion generator paraquat reversed the protective effect of DHEA on the augmented death. The data indicate that DHEA prevents the glucose deprivation-evoked augmented death by inhibiting the production of superoxide anion in immunostimulated C6 glioma cells.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalBrain Research
Issue number2
Publication statusPublished - 2001 Dec 20
Externally publishedYes


  • C6 glioma
  • Dehydroepiandrosterone
  • Glucose deprivation
  • Immunostimulation
  • Peroxynitrite

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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