Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes

Eun Ju Lee; Hyung Jeong Lee; Majid Rasool Kamli; Smritee Pokharel; Abdul R. Bhat; Yong Ho Lee; Bong Hwan Choi; Taehoon Chun; Se Won Kang; Yong Seok Lee; Jae Woo Kim; Robert D. Schnabel; Jeremy F. Taylor; Inho Choi

doi:10.1016/j.ygeno.2012.06.005

Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes

Eun Ju Lee, Hyung Jeong Lee, Majid Rasool Kamli, Smritee Pokharel, Abdul R. Bhat, Yong Ho Lee, Bong Hwan Choi, Taehoon Chun, Se Won Kang, Yong Seok Lee, Jae Woo Kim, Robert D. Schnabel, Jeremy F. Taylor, Inho Choi

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway.

Original language	English
Pages (from-to)	195-202
Number of pages	8
Journal	Genomics
Volume	100
Issue number	3
DOIs	https://doi.org/10.1016/j.ygeno.2012.06.005
Publication status	Published - 2012 Sept

Bibliographical note

Funding Information:
This study was carried out with support from the “ Research Cooperating Program for Agricultural Science & Technology Development (project no. PJ006406 )”, RDA, Republic of Korea and National Research Foundation of Korea, Korean government (MEST) (grant no. 2008‐0060480 ). All research materials used in this study were provided by the Bovine Genome Resources Bank, Yeungnam University, Gyeongsan, Korea.

Keywords

Depots-specific gene expression
Fat
Gene expression
Microarray
Muscle

ASJC Scopus subject areas

Genetics

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10.1016/j.ygeno.2012.06.005

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Lee, E. J., Lee, H. J., Kamli, M. R., Pokharel, S., Bhat, A. R., Lee, Y. H., Choi, B. H., Chun, T., Kang, S. W., Lee, Y. S., Kim, J. W., Schnabel, R. D., Taylor, J. F., & Choi, I. (2012). Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes. Genomics, 100(3), 195-202. https://doi.org/10.1016/j.ygeno.2012.06.005

Lee, EJ, Lee, HJ, Kamli, MR, Pokharel, S, Bhat, AR, Lee, YH, Choi, BH, Chun, T, Kang, SW, Lee, YS, Kim, JW, Schnabel, RD, Taylor, JF & Choi, I 2012, 'Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes', Genomics, vol. 100, no. 3, pp. 195-202. https://doi.org/10.1016/j.ygeno.2012.06.005

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abstract = "We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway.",

keywords = "Depots-specific gene expression, Fat, Gene expression, Microarray, Muscle",

author = "Lee, {Eun Ju} and Lee, {Hyung Jeong} and Kamli, {Majid Rasool} and Smritee Pokharel and Bhat, {Abdul R.} and Lee, {Yong Ho} and Choi, {Bong Hwan} and Taehoon Chun and Kang, {Se Won} and Lee, {Yong Seok} and Kim, {Jae Woo} and Schnabel, {Robert D.} and Taylor, {Jeremy F.} and Inho Choi",

note = "Funding Information: This study was carried out with support from the “ Research Cooperating Program for Agricultural Science & Technology Development (project no. PJ006406 )”, RDA, Republic of Korea and National Research Foundation of Korea, Korean government (MEST) (grant no. 2008‐0060480 ). All research materials used in this study were provided by the Bovine Genome Resources Bank, Yeungnam University, Gyeongsan, Korea.",

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doi = "10.1016/j.ygeno.2012.06.005",

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AU - Lee, Eun Ju

AU - Lee, Hyung Jeong

AU - Kamli, Majid Rasool

AU - Pokharel, Smritee

AU - Bhat, Abdul R.

AU - Lee, Yong Ho

AU - Choi, Bong Hwan

AU - Chun, Taehoon

AU - Kang, Se Won

AU - Lee, Yong Seok

AU - Kim, Jae Woo

AU - Schnabel, Robert D.

AU - Taylor, Jeremy F.

AU - Choi, Inho

N1 - Funding Information: This study was carried out with support from the “ Research Cooperating Program for Agricultural Science & Technology Development (project no. PJ006406 )”, RDA, Republic of Korea and National Research Foundation of Korea, Korean government (MEST) (grant no. 2008‐0060480 ). All research materials used in this study were provided by the Bovine Genome Resources Bank, Yeungnam University, Gyeongsan, Korea.

PY - 2012/9

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N2 - We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway.

AB - We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway.

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Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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