TY - GEN
T1 - Design and application of a DEP-based cell sorter for efficiently separating two types of human MCF cells
AU - An, Jaemin
AU - Kim, Youngho
AU - Kim, Byungkyu
AU - Park, Sukho
AU - Park, Jong Oh
AU - Park, Jungyul
AU - Lee, Sangho
PY - 2006
Y1 - 2006
N2 - This paper presents the development of a novel dielectrophoresis (DEP)-activated cell sorter (DACS) that has 3D-asymmeritc microelectrodes. The DACS conventionally utilizes relative strength between the DEP and drag forces. While the conventional cell separators usually depend on different size of cells, the new system differentiates similarly-sized cells by the difference of the electrical properties among cells. It distinguished different types of cells by their differences in electrical properties, conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enabled us to separate MCF10A cells (normal cells derived from human breast epithelium) and MCF7 cells (human breast cancer cells) that have similar sizes. Applying the newly-developed system to separate two different cells, it was possible to separate cancer cells among normal cells.
AB - This paper presents the development of a novel dielectrophoresis (DEP)-activated cell sorter (DACS) that has 3D-asymmeritc microelectrodes. The DACS conventionally utilizes relative strength between the DEP and drag forces. While the conventional cell separators usually depend on different size of cells, the new system differentiates similarly-sized cells by the difference of the electrical properties among cells. It distinguished different types of cells by their differences in electrical properties, conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enabled us to separate MCF10A cells (normal cells derived from human breast epithelium) and MCF7 cells (human breast cancer cells) that have similar sizes. Applying the newly-developed system to separate two different cells, it was possible to separate cancer cells among normal cells.
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U2 - 10.1109/ICSENS.2007.355508
DO - 10.1109/ICSENS.2007.355508
M3 - Conference contribution
AN - SCOPUS:50149096652
SN - 1424403766
SN - 9781424403769
T3 - Proceedings of IEEE Sensors
SP - 470
EP - 473
BT - 2006 5th IEEE Conference on Sensors
T2 - 2006 5th IEEE Conference on Sensors
Y2 - 22 October 2006 through 25 October 2006
ER -