Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

Min Han; In Su Park; Soo Hyun Kim; Byung Soo Kim; Sang Heon Kim

doi:10.1007/s10529-009-0060-z

Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

Min Han
, In Su Park
, Soo Hyun Kim
, Byung Soo Kim
, Sang Heon Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm² at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.

Original language	English
Pages (from-to)	1677-1684
Number of pages	8
Journal	Biotechnology letters
Volume	31
Issue number	11
DOIs	https://doi.org/10.1007/s10529-009-0060-z
Publication status	Published - 2009 Oct

Bibliographical note

Funding Information:
Acknowledgements This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (M10641000067).

Keywords

Artificial matrix
Cell adhesion
Growth factor
Maltose-binding protein

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-009-0060-z

Cite this

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title = "Design and characterization of a maltose binding protein-linked growth factor for matrix engineering",

abstract = "The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.",

keywords = "Artificial matrix, Cell adhesion, Growth factor, Maltose-binding protein",

author = "Min Han and Park, \{In Su\} and Kim, \{Soo Hyun\} and Kim, \{Byung Soo\} and Kim, \{Sang Heon\}",

note = "Funding Information: Acknowledgements This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (M10641000067).",

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doi = "10.1007/s10529-009-0060-z",

language = "English",

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T1 - Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

AU - Han, Min

AU - Park, In Su

AU - Kim, Soo Hyun

AU - Kim, Byung Soo

AU - Kim, Sang Heon

N1 - Funding Information: Acknowledgements This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (M10641000067).

PY - 2009/10

Y1 - 2009/10

N2 - The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.

AB - The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.

KW - Artificial matrix

KW - Cell adhesion

KW - Growth factor

KW - Maltose-binding protein

UR - https://www.scopus.com/pages/publications/70350271180

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DO - 10.1007/s10529-009-0060-z

M3 - Article

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AN - SCOPUS:70350271180

SN - 0141-5492

VL - 31

SP - 1677

EP - 1684

JO - Biotechnology letters

JF - Biotechnology letters

IS - 11

ER -

Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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