Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

Bishnu Prasad Joshi, Won Mi Cho, Jong Seung Kim, Juyoung Yoon, Keun Hyeung Lee

    Research output: Contribution to journalArticlepeer-review

    69 Citations (Scopus)

    Abstract

    A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

    Original languageEnglish
    Pages (from-to)6425-6429
    Number of pages5
    JournalBioorganic and Medicinal Chemistry Letters
    Volume17
    Issue number23
    DOIs
    Publication statusPublished - 2007 Dec 1

    Bibliographical note

    Funding Information:
    This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

    Keywords

    • Dansyl
    • Fluorescence
    • Metal
    • Peptide sensor
    • Probe
    • Selectivity
    • Zn

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Pharmaceutical Science
    • Drug Discovery
    • Clinical Biochemistry
    • Organic Chemistry

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