Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

Bishnu Prasad Joshi; Won Mi Cho; Jong Seung Kim; Juyoung Yoon; Keun Hyeung Lee

doi:10.1016/j.bmcl.2007.10.008

Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn²⁺ in aqueous solution

Bishnu Prasad Joshi, Won Mi Cho, Jong Seung Kim, Juyoung Yoon, Keun Hyeung Lee

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

A new fluorescent peptide probe for the detection of Zn²⁺ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn²⁺ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

Original language	English
Pages (from-to)	6425-6429
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	23
DOIs	https://doi.org/10.1016/j.bmcl.2007.10.008
Publication status	Published - 2007 Dec 1

Bibliographical note

Funding Information:
This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

Keywords

Dansyl
Fluorescence
Metal
Peptide sensor
Probe
Selectivity
Zn

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2007.10.008

Cite this

@article{b7070181ac694141b7073d19edc241a2,

title = "Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution",

abstract = "A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.",

keywords = "Dansyl, Fluorescence, Metal, Peptide sensor, Probe, Selectivity, Zn",

author = "Joshi, {Bishnu Prasad} and Cho, {Won Mi} and Kim, {Jong Seung} and Juyoung Yoon and Lee, {Keun Hyeung}",

note = "Funding Information: This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.",

year = "2007",

month = dec,

day = "1",

doi = "10.1016/j.bmcl.2007.10.008",

language = "English",

volume = "17",

pages = "6425--6429",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "23",

}

TY - JOUR

T1 - Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

AU - Joshi, Bishnu Prasad

AU - Cho, Won Mi

AU - Kim, Jong Seung

AU - Yoon, Juyoung

AU - Lee, Keun Hyeung

N1 - Funding Information: This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

AB - A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

KW - Dansyl

KW - Fluorescence

KW - Metal

KW - Peptide sensor

KW - Probe

KW - Selectivity

KW - Zn

UR - http://www.scopus.com/inward/record.url?scp=35648931419&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2007.10.008

DO - 10.1016/j.bmcl.2007.10.008

M3 - Article

C2 - 17949975

AN - SCOPUS:35648931419

SN - 0960-894X

VL - 17

SP - 6425

EP - 6429

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 23

ER -