Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution

Bishnu Prasad Joshi; Won Mi Cho; Jong Seung Kim; Juyoung Yoon; Keun Hyeung Lee

doi:10.1016/j.bmcl.2007.10.008

Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn²⁺ in aqueous solution

Bishnu Prasad Joshi, Won Mi Cho, Jong Seung Kim, Juyoung Yoon, Keun Hyeung Lee

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

A new fluorescent peptide probe for the detection of Zn²⁺ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn²⁺ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.

Original language	English
Pages (from-to)	6425-6429
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	23
DOIs	https://doi.org/10.1016/j.bmcl.2007.10.008
Publication status	Published - 2007 Dec 1

Keywords

Dansyl
Fluorescence
Metal
Peptide sensor
Probe
Selectivity
Zn

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2007.10.008

Cite this

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title = "Design, synthesis, and evaluation of peptidyl fluorescent probe for Zn2+ in aqueous solution",

abstract = "A new fluorescent peptide probe for the detection of Zn2+ was synthesized on the basis of zinc binding ligands in zinc enzymes. The peptide that has a unique amino acid sequence displayed a great selectivity for Zn2+ in the presence of several transition metal ions in aqueous solution. The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied. Further, on-bead application of the peptide as chemosensors was demonstrated.",

keywords = "Dansyl, Fluorescence, Metal, Peptide sensor, Probe, Selectivity, Zn",

author = "Joshi, {Bishnu Prasad} and Cho, {Won Mi} and Kim, {Jong Seung} and Juyoung Yoon and Lee, {Keun Hyeung}",

note = "Funding Information: This work was supported by the Grant (R01-2006-000-10956-0) from the BasicResearch Program of the Korea Science & Engineering Foundation and the Grant (C00344) from the basic research program of the Korean Research Foundation. B. P. Joshi and Won-Mi Cho were recipients of a BK21 (II) fellowship.",

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AU - Joshi, Bishnu Prasad

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AU - Yoon, Juyoung

AU - Lee, Keun Hyeung

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KW - Dansyl

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