Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Yoontak Han, Eun Jin Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalJournal of Microbiology
Issue number4
Publication statusPublished - 2020 Apr 1

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2019R1A2C2003460) and by grants from Korea University (K1823071 and K1821661) to EL. The authors declare no conflict of interest.

Publisher Copyright:
© 2020, The Microbiological Society of Korea.


  • Salmonella Typhimurium
  • Type II flagella

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


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