Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Yoontak Han; Eun Jin Lee

doi:10.1007/s12275-020-9297-y

Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Yoontak Han
, Eun Jin Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

Original language	English
Pages (from-to)	245-251
Number of pages	7
Journal	Journal of Microbiology
Volume	58
Issue number	4
DOIs	https://doi.org/10.1007/s12275-020-9297-y
Publication status	Published - 2020 Apr 1

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2019R1A2C2003460) and by grants from Korea University (K1823071 and K1821661) to EL. The authors declare no conflict of interest.

Publisher Copyright:
© 2020, The Microbiological Society of Korea.

Keywords

Salmonella Typhimurium
Type II flagella

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.1007/s12275-020-9297-y

Cite this

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title = "Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry",

abstract = "The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.",

keywords = "Salmonella Typhimurium, Type II flagella",

author = "Yoontak Han and Lee, \{Eun Jin\}",

note = "Funding Information: This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT \& Future Planning (NRF-2019R1A2C2003460) and by grants from Korea University (K1823071 and K1821661) to EL. The authors declare no conflict of interest. Publisher Copyright: {\textcopyright} 2020, The Microbiological Society of Korea.",

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doi = "10.1007/s12275-020-9297-y",

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N2 - The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

AB - The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

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KW - Type II flagella

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Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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