TY - JOUR
T1 - Detection and imaging of cathepsin L in cancer cells using the aggregation of conjugated polymer dots and magnetic nanoparticles
AU - Kim, Daigeun
AU - Lee, Yong Deok
AU - Jo, Seonyoung
AU - Kim, Sehoon
AU - Lee, Taek Seung
N1 - Funding Information:
This work was supported by the National Research Foundation (NRF) of Korean government through Basic Science Research Program ( 2018R1A2A2A14022019 ).
Funding Information:
This work was supported by the National Research Foundation (NRF) of Korean government through Basic Science Research Program(2018R1A2A2A14022019).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/3/15
Y1 - 2020/3/15
N2 - New technique for cathepsin L detection and imaging was developed via controlling an aggregation between magnetic nanoparticles (MNPs) and conjugated polymer dots (CPdots). By changing the electrostatic surface charges of MNPs, the techniques provided two protocols: 1) fluorescence quenching and spin-spin relaxation (T2)-based magnetic resonance imaging (MRI); 2) fluorescence recovery for fluorescent imaging. By adding cathepsin L, the fluorescence and MRI relaxivity were altered by the formation of aggregates of CPdots and MNPs, inducing fluorescence quenching by MNP as well as a change in T2 relaxation. In addition, nonfluorescent, pre-aggregated nanohybrid (CPdots and MNPs) can be dissolved in the presence of cathepsin L, resulting in turn-on fluorescent detection and the imaging of cathepsin L in cancer cells and in mice with tumors. In all cases, the MNPs played important roles as the fluorescence quencher and as the MRI-signal generator. The surface of CPdots was covered with poly(L-lysine) (pLys) for the aggregation of CPdots and MNPs, in which pLys was specifically degraded by the enzymatic action of cathepsin L. The CPdots systems showed high sensitivity and selectivity toward cathepsin L, by which these systems can be considered as potential candidates for cathepsin L detection and imaging.
AB - New technique for cathepsin L detection and imaging was developed via controlling an aggregation between magnetic nanoparticles (MNPs) and conjugated polymer dots (CPdots). By changing the electrostatic surface charges of MNPs, the techniques provided two protocols: 1) fluorescence quenching and spin-spin relaxation (T2)-based magnetic resonance imaging (MRI); 2) fluorescence recovery for fluorescent imaging. By adding cathepsin L, the fluorescence and MRI relaxivity were altered by the formation of aggregates of CPdots and MNPs, inducing fluorescence quenching by MNP as well as a change in T2 relaxation. In addition, nonfluorescent, pre-aggregated nanohybrid (CPdots and MNPs) can be dissolved in the presence of cathepsin L, resulting in turn-on fluorescent detection and the imaging of cathepsin L in cancer cells and in mice with tumors. In all cases, the MNPs played important roles as the fluorescence quencher and as the MRI-signal generator. The surface of CPdots was covered with poly(L-lysine) (pLys) for the aggregation of CPdots and MNPs, in which pLys was specifically degraded by the enzymatic action of cathepsin L. The CPdots systems showed high sensitivity and selectivity toward cathepsin L, by which these systems can be considered as potential candidates for cathepsin L detection and imaging.
KW - Cathepsin L
KW - Conjugated polymer dots
KW - Fluorescence
KW - Imaging
KW - Magnetic nanoparticles
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=85078718244&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.127641
DO - 10.1016/j.snb.2019.127641
M3 - Article
AN - SCOPUS:85078718244
SN - 0925-4005
VL - 307
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 127641
ER -