TY - JOUR
T1 - Detection of Cd2+ and Pb2+ using amyloid oligomer–reduced graphene oxide composite
AU - Kim, Chihyun
AU - Park, Joohyung
AU - Kim, Woochang
AU - Lee, Wonseok
AU - Na, Sungsoo
AU - Park, Jinsung
N1 - Funding Information:
This work was supported by the National Research Foundation of South Korea (NRF) [grant numbers NRF-2017R1E1A1A01075439, NRF-2022R1A2C4001990, and NRF-2022R1I1A1A01066196]; the Ecological Imitation-Based Environmental Pollution Management Technology Development Project, South Korea Environment Industry and Technology Institute, South Korea Ministry of Environment (2019002800009); the Korea Medical Device Development Fund, Ministry of Science and ICT, South Korea [project number 202012D19]; and “Regional Innovation Strategy (RIS)”, National Research Foundation of Korea (NRF), Ministry of Education (MOE) (2021RIS-001(1345341783).
Publisher Copyright:
© 2022
PY - 2022/10
Y1 - 2022/10
N2 - Heavy metal ions are toxic to humans and can further interact with amyloid in the human body to produce amyloid plaques, which disrupt neurotransmitter function and are linked to Alzheimer's and Parkinson's diseases. In this study, we developed an amyloid oligomer–reduced graphene oxide composite (AOrGOC) electrochemical sensor for effective heavy metal ion detection based on square-wave anodic stripping voltammetry. The reactivity between amyloids and heavy metal ions was studied by analyzing the stripping current for different amyloids (lysozyme, bovine serum albumin, and β-lactoglobulin) and amyloid growth types (monomers, oligomers, and fibrils). Reduced graphene oxide was used to improve the sensitivity of the sensor. The AOrGOC sensor exhibited the detection limits of 86.0 and 9.5 nM for Cd2+ and Pb2+, respectively, and selectively detected Cd2+ and Pb2+ over other heavy metal ions. The AOrGOC sensor also detected Cd2+ and Pb2+ in human plasma, thus exhibiting its potential as a biosensor. This study not only promoted our fundamental understanding of amyloids and the detection of heavy metal ions using amyloids, but also provided valuable insights into amyloid-based electrochemical sensors.
AB - Heavy metal ions are toxic to humans and can further interact with amyloid in the human body to produce amyloid plaques, which disrupt neurotransmitter function and are linked to Alzheimer's and Parkinson's diseases. In this study, we developed an amyloid oligomer–reduced graphene oxide composite (AOrGOC) electrochemical sensor for effective heavy metal ion detection based on square-wave anodic stripping voltammetry. The reactivity between amyloids and heavy metal ions was studied by analyzing the stripping current for different amyloids (lysozyme, bovine serum albumin, and β-lactoglobulin) and amyloid growth types (monomers, oligomers, and fibrils). Reduced graphene oxide was used to improve the sensitivity of the sensor. The AOrGOC sensor exhibited the detection limits of 86.0 and 9.5 nM for Cd2+ and Pb2+, respectively, and selectively detected Cd2+ and Pb2+ over other heavy metal ions. The AOrGOC sensor also detected Cd2+ and Pb2+ in human plasma, thus exhibiting its potential as a biosensor. This study not only promoted our fundamental understanding of amyloids and the detection of heavy metal ions using amyloids, but also provided valuable insights into amyloid-based electrochemical sensors.
KW - Amyloid
KW - Cadmium ion
KW - Electrochemical sensor
KW - Heavy metal ion
KW - Lead ion
KW - Square Wave Anodic Stripping Voltammetry (SWASV)
UR - http://www.scopus.com/inward/record.url?scp=85134829436&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2022.108214
DO - 10.1016/j.bioelechem.2022.108214
M3 - Article
C2 - 35901626
AN - SCOPUS:85134829436
SN - 1567-5394
VL - 147
JO - Bioelectrochemistry
JF - Bioelectrochemistry
M1 - 108214
ER -