Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

Seung Heon Lee; Young Kil Park; Sung Weon Ryo; Myung Sup Shim; Woo Jin Lew; Hee Jin Kim

doi:10.4046/trd.2008.64.6.422

Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

Seung Heon Lee, Young Kil Park, Sung Weon Ryo, Myung Sup Shim, Woo Jin Lew, Hee Jin Kim

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.

Original language	English
Pages (from-to)	422-426
Number of pages	5
Journal	Tuberculosis and Respiratory Diseases
Volume	64
Issue number	6
DOIs	https://doi.org/10.4046/trd.2008.64.6.422
Publication status	Published - 2008 Jun
Externally published	Yes

Keywords

23S rRNA
Broth microdilution test
Clarithromycin
Mycobacterium abscessus
Reverse hybridization assay

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4046/trd.2008.64.6.422

Cite this

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title = "Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus",

abstract = "Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.",

keywords = "23S rRNA, Broth microdilution test, Clarithromycin, Mycobacterium abscessus, Reverse hybridization assay",

author = "Lee, {Seung Heon} and Park, {Young Kil} and Ryo, {Sung Weon} and Shim, {Myung Sup} and Lew, {Woo Jin} and Kim, {Hee Jin}",

year = "2008",

month = jun,

doi = "10.4046/trd.2008.64.6.422",

language = "English",

volume = "64",

pages = "422--426",

journal = "Tuberculosis and Respiratory Diseases",

issn = "1738-3536",

publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",

number = "6",

}

TY - JOUR

T1 - Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

AU - Lee, Seung Heon

AU - Park, Young Kil

AU - Ryo, Sung Weon

AU - Shim, Myung Sup

AU - Lew, Woo Jin

AU - Kim, Hee Jin

PY - 2008/6

Y1 - 2008/6

N2 - Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.

AB - Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.

KW - 23S rRNA

KW - Broth microdilution test

KW - Clarithromycin

KW - Mycobacterium abscessus

KW - Reverse hybridization assay

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U2 - 10.4046/trd.2008.64.6.422

DO - 10.4046/trd.2008.64.6.422

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AN - SCOPUS:47149108345

SN - 1738-3536

VL - 64

SP - 422

EP - 426

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

IS - 6

ER -

Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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